Auflistung nach Autor "Friedhoff, Peter"
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Biochemical characterization of the nidoviral replicase complex
Bylapudi, Ganesh (2021-07)Nidoviruses are a monophyletic group of RNA viruses with exceptionally large genomes ranging from 12 - 41 kb. Despite their widely varying genome sizes, members of the order Nidovirales share common characteristic features, ... -
Biotechnologische Darstellung und analytische Charakterisierung aromaaktiver Fettaldehyde
Hammer, Andreas Klaus (2021)Eine große Bandbreite an Geruchsqualitäten und vergleichsweise niedrige Geruchsschwellen machen Aldehyde für die Aromaindustrie zu einer interessanten Substanzklasse. Trotz diverser Lösungsansätze fehlt allerdings nach wie ... -
Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3
Schneider, Tim; Hung, Lee-Hsueh; Aziz, Masood; Wilmen, Anna; Thaum, Stephanie; Wagner, Jaqueline; Janowski, Robert; Müller, Simon; Schreiner, Silke; Friedhoff, Peter; Hüttelmaier, Stefan; Niessing, Dierk; Sattler, Michael; Schlundt, Andreas; Bindereif, Albrecht (2019)How multidomain RNA-binding proteins recognize their specific target sequences, based on a combinatorial code, represents a fundamental unsolved question and has not been studied systematically so far. Here we focus on a ... -
Covalent and kinetic trapping repair machinery for structural and functional studies
Kunetsky, Vladislav (2021)The principal attribute of living matter is genetic information used for reproduction. Before the cell can divide, all genetic information must be reproduced without errors leading to mutations. To decrease the number of ... -
Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling
Heinze, Roger J.; Sekerina, Svetlana; Winkler, Ines; Biertuempfel, Christian; Oretskaya, Tatiana S.; Kubareva, Elena; Friedhoff, Peter (2012)The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a ... -
Function of the RNA helicase Sub2 from Saccharomyces cerevisiae and its regulation by Tho1
Miosga, Matthias Bastian (2022-07)In eukaryotes, the transcription of mRNA takes place in the nucleus of each cell. Already during transcription, various proteins bind the mRNA to prepare it for export from the nucleus so that the information of the genome ... -
NADPH-producing Enzymes from the Pentose Phosphate Pathway of Plasmodium and Leishmania as Targets for New Anti-infective Agents
Berneburg, Isabell (2023-06)Malaria and the so-called neglected tropical diseases (NTDs) such as leishmaniasis, Chagas disease, and schistosomiasis remain a major public health problem that disproportionately affects the most vulnerable populations ... -
Secondary 3-Chloropiperidines: Powerful Alkylating Agents
Georg, Mats; Laping, Lina Alexandra; Billo, Veronica; Gatto, Barbara; Friedhoff, Peter; Göttlich, Richard (2023)In previous works, we demonstrated that tertiary 3-chloropiperidines are potent chemotherapeutics, alkylating the DNA through the formation of bicyclic aziridinium ions. Herein, we report the synthesis of novel secondary ... -
Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA
Cristovao, Michele; Sisamakis, Evangelos; Hingorani, Manju M.; Marx, Andreas D.; Jung, Caroline P.; Rothwell, Paul J.; Seidel, Claus A. M.; Friedhoff, Peter (2012)Mismatch repair (MMR) corrects replication errors such as mismatched bases and loops in DNA. The evolutionarily conserved dimeric MMR protein MutS recognizes mismatches by stacking a phenylalanine of one subunit against ... -
Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats
Gabsalilow, Lilia; Schierling, Benno; Friedhoff, Peter; Pingoud, Alfred; Wende, Wolfgang (2013)Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by ... -
The role of effector proteins on suppression of the plant immune system : a case study: ‘HaRxL21’
Kumari, Priyanka (2022)Plant diseases result from the interaction between the pathogen and the host plant, where pathogens deploy effector proteins to invade the host immune system to aid colonization. The oomycete Hyaloperonospora arabidopsidis ... -
Use of Rna-based technologies for targeted gene silencing in cereals
Galli, Matteo (2022-02)Gene silencing techniques are essential to study gene function and produce crops with desired agronomic traits. Over the past decade, RNA interference (RNAi)-based methods involving experimental modulation of gene expression ...