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dc.contributor.authorAlbuquerque, Wendell
dc.contributor.authorGhezellou, Parviz
dc.contributor.authorSeidel, Leif
dc.contributor.authorBurkert, Johannes
dc.contributor.authorWill, Frank
dc.contributor.authorSchweiggert, Ralf
dc.contributor.authorSpengler, Bernhard
dc.contributor.authorZorn, Holger
dc.contributor.authorGand, Martin
dc.date.accessioned2023-04-13T11:04:13Z
dc.date.available2023-04-13T11:04:13Z
dc.date.issued2023
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/16190
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-15572
dc.description.abstractThe comprehensive identification of the proteome content from a white wine (cv. Silvaner) is described here for the first time. The wine protein composition isolated from a representative wine sample (250 L) was identified via mass spectrometry (MS)-based proteomics following in-solution and in-gel digestion methods after being submitted to size exclusion chromatographic (SEC) fractionation to gain a comprehensive insight into proteins that survive the vinification processes. In total, we identified 154 characterized (with described functional information) or so far uncharacterized proteins, mainly from Vitis vinifera L. and Saccharomyces cerevisiae. With the complementarity of the two-step purification, the digestion techniques and the high-resolution (HR)-MS analyses provided a highscore identification of proteins from low to high abundance. These proteins can be valuable for future authentication of wines by tracing proteins derived from a specific cultivar or winemaking process. The proteomics approach presented herein may also be generally helpful to understand which proteins are important for the organoleptic properties and stability of wines.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG); ROR-ID:018mejw64
dc.language.isoen
dc.rightsNamensnennung 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSilvaner
dc.subjectproteomics
dc.subjectwine
dc.subjectproteins
dc.subjectmass spectrometry
dc.subjectVitis vinifera
dc.subject.ddcddc:540
dc.titleMass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine
dc.typearticle
local.affiliationFB 08 - Biologie und Chemie
local.projectINST 162/500-1 FUGG
local.source.spage1
local.source.epage18
local.source.journaltitleBiomolecules
local.source.volume13
local.source.articlenumber650
local.source.urihttps://doi.org/10.3390/biom13040650


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