marine drugs Article A New Citrinin Derivative from the Indonesian Marine Sponge-Associated Fungus Penicillium citrinum Aninditia Sabdaningsih 1,2,3,4,5,* , Yang Liu 3,6,*, Ute Mettal 3,6, John Heep 6 , Riyanti 3,6,7, Lei Wang 3,6, Olvi Cristianawati 1,4,5, Handung Nuryadi 4,8, Mada Triandala Sibero 4,5,9, Michael Marner 6 , Ocky Karna Radjasa 4,9,10, Agus Sabdono 4,9 , Agus Trianto 5,9 and Till F. Schäberle 3,6,11,* 1 Department of Coastal Resources Management, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50275, Indonesia; olvi.cristiana@yahoo.com 2 Department of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50275, Indonesia 3 Institute for Insect Biotechnology, Justus-Liebig-University of Giessen, 35392 Giessen, Germany; Ute.Mettal@chemie.uni-giessen.de (U.M.); riyanti@bio.uni-giessen.de or riyanti.anti@gmail.com (R.); Lei.Wang@agrar.uni-giessen.de (L.W.) 4 Tropical Marine Biotechnology Laboratory, Diponegoro University, Semarang 50275, Indonesia; handung.nuryadi87@gmail.com (H.N.); madatriandala@hotmail.com (M.T.S.); ocky_radjasa@yahoo.com (O.K.R.); agus_sabdono@yahoo.com (A.S.) 5 Marine Natural Product Laboratory, Diponegoro University, Semarang 50275, Indonesia; agustrianto.undip@gmail.com 6 Department of Bioresources of the Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany; John.Heep@ime.fraunhofer.de (J.H.); Michael.Marner@ime.fraunhofer.de (M.M.) 7 Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53122, Indonesia 8 Graduate School of Engineering and Science, University of the Ryukyus, 1 Senbaru, Nihihara, Okinawa 903-0213, Japan 9 Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50275, Indonesia 10 Ministry of Research and Technology of the Republic of Indonesia, Jakarta 10340, Indonesia 11 German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35392 Giessen, Germany * Correspondence: aninditiasabdaningsih@live.undip.ac.id (A.S.); Liu.Yang@agrar.uni-giessen.de (Y.L.); till.f.schaeberle@agrar.uni-giessen.de (T.F.S.); Tel.: +49-641-99-37140 (T.F.S.)  Received: 2 April 2020; Accepted: 20 April 2020; Published: 24 April 2020  Abstract: Sponge-associated fungi are attractive targets for the isolation of bioactive natural products with different pharmaceutical purposes. In this investigation, 20 fungi were isolated from 10 different sponge specimens. One isolate, the fungus Penicillium citrinum strain WK-P9, showed activity against Bacillus subtilis JH642 when cultivated in malt extract medium. One new and three known citrinin derivatives were isolated from the extract of this fungus. The structures were elucidated by 1D and 2D NMR spectroscopy, as well as LC-HRMS. Their antibacterial activity against a set of common human pathogenic bacteria and fungi was tested. Compound 2 showed moderate activity against Mycobacterium smegmatis ATCC607 with a minimum inhibitory concentration (MIC) of 32 µg/mL. Compound 4 exhibited moderate growth inhibition against Bacillus subtilis JH642, B. megaterium DSM32, and M. smegmatis ATCC607 with MICs of 16, 16, and 32µg/mL, respectively. Furthermore, weak activities of 64 µg/mL against B. subtilis DSM10 and S. aureus ATCC25923 were observed for compound 4. Keywords: antibacterial; citrinin derivatives; marine-derived fungi; Penicillium citrinum; penicitrinone; marine sponges Mar. Drugs 2020, 18, 227; doi:10.3390/md18040227 www.mdpi.com/journal/marinedrugs Mar. Drugs 2020, 18, 227 2 of 12 1. Introduction The discovery of bioactive compounds from the marine environment has increased over the last two decades, showing the potential of this habitat for the discovery of novel structures [1,2]. As an example, marine sponges are a reported source for agents with diverse biological activities, e.g., antimicrobial or cytostatic activity [3–8]. However, in order to conduct preclinical and clinical trials and to develop a promising hit compound into a lead candidate for a marketed drug, continuous substance supply is crucial. For instance, one kilogram wet weight of the sponge Lissodendoryx sp. yielded only 400 µg of halichondrin B [9]. Only chemical synthesis of the complex natural product enabled further development as a lead structure, which finally led to the synthesis of the structurally simplified and pharmaceutically optimized analog eribulin [10]. Furthermore, sponges have an important role in their ecosystems [11]. Therefore, exploitation of this bioresource must be avoided. Hence, for compounds that are produced by sponge-associated microorganisms, e.g., fungi, it would be desirable to cultivate the latter. In that way, the supply issue should be easier to solve, since fermentation can be done in a more sustainable fashion under laboratory conditions rather than harvesting the slow-growing macroorganisms. Such fungi were shown to produce a wide range of chemically diverse compounds, displaying a variety of biological activities, e.g., antibacterial, anti-inflammatory, antiviral, and anticancer activity [12–17]. The antibacterial compounds aspochalasin B and D were successfully isolated from a crude extract of Aspergillus flavipes. This fungus was associated with a sponge of the Demospongiae class, and the fungal extract exhibited growth inhibition of Bacillus subtilis, Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) [18]. Moreover, the fungus Neosartorya fennelliae KUFA 0811, associated with the marine sponge Clathria reinwardtii and a producer of the antibacterial agents paecilin E and dankastetrone A. Paecilin E, showed activity against S. aureus ATCC 29213 and Enterobacter faecalis ATCC 29212, while dankastetrone A was active against E. faecalis ATCC 29212 and the multidrug-resistant VRE Enterobacter faecalis A5/102 [19]. Thus, compounds from sponge-associated fungi already proved to be highly valuable for the detection of interesting biological and pharmaceutical activities and it is expected that many more new natural products are waiting to be discovered. Considering promising bioresources, one valid approach is to select species originating from challenging environments, e.g., habitats with a high biodiversity. The coral triangle which has rich biodiversity and the greatest repository of marine life, represents such an environment [20]. Therefore, we report herein, the sponge collection from the Wakatobi National Park, located in South East Sulawesi, Indonesia. Associated fungi were retrieved, molecules were isolated based on bioactivity, their structures elucidated, and their antibacterial activities tested. 2. Results 2.1. Sample Collection, Isolation, and Structure Elucidation During our ongoing research to discover antibacterial compounds, 10 sponges were collected from Hoga Island located in Wakatobi National Park (Figure 1). This region was classified as a Marine Protected Area in 2002 [21,22]. Between 1993 and 2013, 112 marine natural products have been isolated from various organisms, and at least 51 compounds have been reported as a pharmaceutical agent [23]. From our 10 sponge samples, 20 fungal colonies were isolated, mainly belonging to the genera Aspergillus and Penicillium. Initial activity screenings of those 20 fungi were performed and the crude extract of Penicillium citrinum WK-P9, isolated from a Suberea sp., showed antibacterial activity against Bacillus subtilis and Bacillus megaterium, which prompted us to isolate the corresponding specialized metabolites. The ethyl acetate (EtOAc) extract of the sponge-derived fungus P. citrinum WK-P9 cultivated in malt extract broth medium was fractionated by medium pressure liquid chromatography (MPLC) with reverse phase silica gel C18. The active fractions obtained by bioassay-guided fractionation were further purified by size exclusion chromatography and by HPLC. This procedure led to the isolation of one new citrinin derivative (1), and 3 known compounds, namely, penicitrinone A (2), penicitrinone E (3), penicitrinol J (4) (Figure 2). Mar. Drugs 2020, 18, 227 3 of 12 MMara. rD. Drurgusg 2s 021091,9 1, 71,7 x, xF FOORR P PEEEERR R REEVVIEIEWW 33 oof f 1122 FFiiFggiuugrureer e11 ..1 a.a ::a :SS Saaammppplliilnninggg ss siittieete aa att tW Waaakkkaaattotoobbbiii N Naaatttiioioonnnaaalll PPPaaarrrkkk;;; bb::: uunnddeeerrrwwaaattteeerrr ppiiiccctttuurrreee ooofff sssppooonngggeee SSSuuubbbeeerrreeeaaa sssppp.. . WWKK-K-PP-9P9; ;9c ;:c :cp :pi cpitcuitcurteureroe fo tofh fet htthehe e trhteheferroreemfrfrooimsmo l iaistsoeoldlaatfteuednd g ffuuusnngPgueunssi cPPileelninuiicmciilllcliiiuutrmmin ucciimttrriiWnnuuKmm-P WW9,KKg-r-PoPw99,, n ggorronowwannm oaonlnt aeax mtmraaacllttt aegxeatxrrtarpcaltca tat ega.gara rp plalatete. . Figure 2. Structures of the isolated compounds from sponge-associated fungus P. citrinum WK-P9. Figure 2.. Structures of the isolated compounds from sponge--associiatted ffunguss P..c cititrrininuumm WKK--PP99.. Compound 1 was isolated as a white amorphous solid with an optical rotation value of α 24D24= 1264.2 C5 o(mc 0p.o0u3,n Md 1eO wwHaa)s.s iIstiossl oamltaeotdele dacsua lasa rw afhowirtmeh iautmelao awrmpahos orepushsta osbuolsliisdhs eowdlii dtahs w aCnit ho22Hpatin24Ocao,l pbrotaitscaeatdlio ornon vt atahtliueo enp orvof amlαuineDeno=f 6 t [1α6p].Ds2e5u= (dc1o 60m..20o53l,e (cMcul0eaO.r0 3Hio,)n.M IpteseO amHkso) .laeItc tumsl/amzr o3fl8oe5rc.mu16lua5lr3a f[woMram s+ u eHlsat]a+ wbalnaissdh e4sd0t 7aa.b1sl4 iC8s9h22 eH[dM24 aO+s 6,NC ba2a2]+sH eidn24 Otohn6e, tLbhCaes -peHdrRoEmonSiInMtehnSet pprssopemuecditnoreumnmot lp(eFcsiueguluadrreo i moS7no) l.p eTechuaekla s1r Haito mn /pz e3a8k5s.1a6t53m /[zM3 8+5 .H1 + NMR spectrum of 1 re6]v5e 3aanl[edM + d 4t+w07oH.1 o4]x8y9am n[Medth 4+i0 N7.a1]4 + ne pr8o i9tn + 1 o [ntMsh e(H+ L-CN1,- aHδ] 4R.E4in3S;I tMHhe-S LspC1e′-,Hc δtrR u4E.m3S7 I)(M 1 ,F fiSgouusprr em cSte7rt)uh. miTnhe(e Fp irgHout rNoenMSs 7R()H .s-Tp6he′,c eδtr u3H.m7N1 o; MfH 1R- 6r,se pδv e3ca.t6lre3ud;m Htwo-2of, 1oδx r2ey.vm9e9ea; tlHhedi-n2te′w, pδo r2oo.t9xo1yn)m,s a(eHnthd-i1 ns, ieδx p4mr.4oe3ttoh; nHysl- (1H′g, -rδ1o ,4δ.347.)4,3 f;oHur- 1m ′ ups (H-10’, δ, e1δt.3h46i.n;3 eH7 )-p,1rf1o’ut, oδrn m1s. 3e(3Hth2-;i6 nH′,e -δ1p 13r,o. 7δt1o 1;n .3Hs2(-56H;, H-δ6 ′ -39, .’δ6, 3δ3; . 17H.13-;02;H, Hδ-6 -29, .,δ9 δ93 ;1. 6.H234-;2; H′H, -δ-21 ,20δ.,9 δ21 .)19,. 92a;2n)Hd ′ ( -Fs2iigx,u δmre2e .St9h11)y).,l agnrTdohusepi x1s3C m(H eNt-hM1y0R’l, gδsrp o1eu.c3pt6rs;a (H ′ Hsu--1g11g0’e, ,sδδt e11d..3 3f362; ′ ou;H rH -c1-a1r1b,,o δn 1y..3l3 3g22r5;o; uHp--1s91 ’(,,C δ- 51..3 ′ , δ20 15; 9;H9.-5-99;, Cδ, - δ151.′,2 .3δ40 ;1 ;H9H9-1.-090;, , Cδδ- 171.,.2 2δ42 ;1)H 8(F4-1.i7g0;u, Cδre-17 .S′2,1 2δ)) . (TF1hig8eu4 1.r32Ce), S Nt1wM). RT hsepe 13 o oxygcetCnraaNt esMdu gsRgpe2s spctaercdbt orfaonuss ru( Cgcag-8reb,s oδte n1dy5l0f og.3ur;o rCuc-pa8sr′, b (δCo n1-55y,0l δ.g8 1)r,o9 tu9w.p5os; Cs(pC-25- ′5c,a ,δrδ b11o9n99s.0. 5(; C;CC--37-,5 , ′ δδ , 11δ48014.90.79; ;. C0C;--3C7′,′- ,7δ δ, δ181 ′ 21483.942..)27,) ;,tC wfo-o7u ro, xsδpy13g 8ce4an.r2ab)to,entdws siopn2o pcxrayorgbxeiomnnasitt ye(Cd to-s8 pa, nδc e1al5reb0c.ot3rn;o snC (-wC8′-i,t8 hδ, ′ dδ1r51a05w.08i.)n3, g;t Cwg-ro8o us,ppδ2 1(cC5a0r-1b.8,o )δn, s8tw 9(.Co 2 2;- s3Cp, -δ1c ’1,a 4δr0b 8.o08n;. 3sC; (-CC3-′-,63 ,δ, δ131δ94 ′ 3 7.023.)0.1,; ;fC oC-u3-6r ,′s, δpδ3 1 7c31a9.r.92b)),o, anfnosuo itnrh seprr ofcoxauirmrb ositpny3s ctioanr abpnor onexslei m(cCtir-to4y,n tδ ow 5ai5nt.h6ed; lCerac-tw4r′o,i nδg 5w 5gi.rt1ho; duCrp-a 2w(′C, iδ-n1 g4, 7δg. 1r8o, 9uC.2p-;2 (C-- ′ , δ 14’,5, δ.δ3 8)8,9 8a.2.n3;d;C C s-1-ix6, , δδ 8m783e.3.t1;h;C yCl- 6-g6,r′δ,o δ7u 3p7.1s .;(9CC),-- 6a ′n, 39’, oδδt 7h210e..r97 )f;, oCaun-r9o ,st phδ3 e 2cr0af.ro6bu; oCrns-sp1 0(C,c δa- 4r2b, 0oδ.3n 5;s 5C(.C6-;1- 04C,’-,δ 4δ′5, 15δ9. 65.3;5;C .1C-;4- C ′ 1,1-δ,2 5δ′, 5δ1.1 64;.71C;. 1-C2, ′ -C,1δ-12’4,, 7δδ. 141,54C..53-))2 ,,( aFδni4gd5u .sr3ie)x, amnSed2t)hs. iyFxl mgreot ′ ′ ′ urtheuhrpy asln g(aCrloy-9us’ip,s sδo (f2C t0h-.97e; 1,CDδ- 92a,0n .δd7 ;2 20CD.-6 9N; ,CMδ-12R0 ,d. 6δa; tC2a0,- .1a30l;l ,oCδw-21e0d’. 3,f ;oδC r1 t-9h1.e30 ;a ,Csδs-i1g19n,. 3mδ; e1Cn6-t.1 1o1;f ,C tδw-1o16 ’.i,1d ;δe Cn1-t4i1.c51a)l , (pδFlia1gn4u.a5rre) S2m).o Fieutrieths.e Tr hane aulnyesiqsu oivf othcael 1 aDss aignndm 2eDn tNs MofR th dea 1tHa, aanlldow 13eCd N foMr Rth dea atsas figorn meaecnht uonf ittw woe irde ebnatsiecadl opnla tnhaer moieties. The unequivocal assignments of the 1H and 13C NMR data for each unit were based on the Mar. Drugs 2020, 18, 227 4 of 12 (Figure S2). Further analysis of the 1D and 2D NMR data, allowed for the assignment of two identical Mplaarn. Darumgs o20ie19ti, e1s7., xT FhOeRu PnEeEqRu RivEVoIcEaWl a ssignments of the 1H and 13C NMR data for each unit were 4b aosf e1d2 on the analysis of the COSY, heteronuclear single quantum correlation (HSQC), and heteronuclear amnualltyispilse obfo tnhde CcoOrrSeYla, thioetner(HonMucBlCea)rs psiencgtrlea q(Tuaabnlteu1m, F ciogrurreelastSio3n–S (5H).SQC), and heteronuclear multiple bondI ncotrhreelCatOioSnY (HspMecBtCru)m sp, etwctroas (pTianbsley s1t,e Fmigsuarree So3b–sSe5r)v. e d. One of them consists of H-9, H-1, H-2, and IHn- 1th0e, wCOhiSleY tshpeecottrhuemr ,o tnweoc ospminp rsiyssetsemHs-9 a′,reH o-b1s′,erHv-e2d′., Oannde oHf -t1h0e′m. Tchoinssiisstsa losfo Hco-9n, fiHrm-1e, dH-b2y, aHnMd BHC-1c0o, rwrehlialtei othnes .oFthoerrt hoenefi crostmmporilseecsu Hlar-9f’r, aHg-m1’e, nHt,-2th’,e aHndM HB-C10c’o. Trrheilsa tiiso anlssor acnognefirfrmoemd Hby-1 HtoMCB-C3 caonrdreCla-t8i,ofnrso.m FoHr t-h2et ofirCst- 3m, Col-e8c,ualnard frCa-g1m0,efnrot,m thHe H-9MtoBCC -c1o,rCre-l2a,taionnds frraonmgeH fr-o1m0 t Ho -C1- t1o, CC--32 aanndd CC--83,. fCroomrre sHp-o2n dtoin Cgl-y3,, thCe-8s,e caonndd Cfr-a1g0m, efrnotmsh oHw-9s HtoM CB-C1, cCor-r2e, laatniodn sfrformom HH-1-10′ ttoo CC--31′, aCnd-2 Ca-n8′d, fCro-m3. CHo-2rr′etsopCon-3d′,inCg-l8y′,, athned sCec-1o0n′d, ffrroamgmHe-n9t′ tsohoCw-1s′ ,HCM-2B′,Ca ncdorfrreolmatiHon-1s 0f′rotomC H-1-′1, ’C t-o2 ′Ca-n3d’ aCn-d3 ′C. -8’, from H-2’ Ftou rCth-3e’r, CH-M8’B, aCncdo Crr-e1la0t’,i ofnrosmfr oHm-9H’ t-o6 Cto-1C’-, 5C, -C2-’7, ,aanndd frCo-m8, aHs-w10e’l tloa sCt-h1e’, cCo-r2r’e laantido nCs-3fr’.o m H-11 to C-3, CFu-4r,thCe-r5 ,HaMndBCC- c8oersrtealbaltiisohnesd frtohme fi Hrs-t6 mtoo Cno-5m, Cer-i7c, uannidt aCs-8sh, aosw wneilnl aFsig tuhree c3or(brelalactkio).nAs cfrcoomrd iHng-1ly1, ttoh eCse-3c,o nCd-4m, oCn-o5m, earnicd uCni-t8( Feisgtuabreli3sh, gerde yt)hies cfoirnsfit rmoednobmy eHriMc BuCnicto rarse lasthioonwsnf roinm FHig-6u′rteo 3C -(5b′,laCc-k7)′., AancdcoCrd-8in′,galsy,w tehlel assectohnedc omrroenlaotmioenrsicf ruonmitH (F-1ig1u′ rtoe C3,- 3g′r,eCy)-4 i′s, Cco-n5′f,iramndedC b-8y′ .HMBC correlations from H-6’ Atos Cc-a5n’, bCe-7co’,n acnludd Ce-d8’f,r aosm wtehlel aHsM thBeC cocrorrerlealtaitoionns sfrforomm HH-1-11’1 ttoo CC--36’′, ,Cfr-o4’m, CH-5-6’, taondC -C3′-,8C’. -4′ and C-11′A, fsr coamn Hbe- 1c1o′ntcoluCd-6e,da fnrdomfr othme HH-M6′BtoC Cco-3r,reCl-a4tiaonnds fCro-1m1, Hth-e11tw too Cm-6o’n, ofrmoemri Hc u-6n ittos Car-e3’c, oCn-n4e’ catnedd Cvi-a11b’o, nfrdosmb eHtw-1e1e’ ntoC C-4-6a, nadndC f-r6o′,ma sHw-6e’l ltoa sCC-3-6, Ca-n4d aCnd-4 C′. -T1h1,e trheefo trwe,oc momonpoomunedric1 uwnaistse alurec icdoantendecatseda vnieaw bonnatdusr baletpwroedenu cCt,-4fo arnwdh Cic-h6’,w aes pwreolpl oasse Ct-h6e annadm Ce-p4′e. nTihcietrreinfoornee, cGo.mpound 1 was elucidated as a new nTahteurrealla ptirvoeducocnt,fi fogru wrahtiiocnh wwae spdroepteorsme itnheed nbaamseed peonnicNitOriEnoannea Gly.s is together with the coupling constTahnet .reTlhateivceo rcroenlafitgiounrastfiroonm wHas- 6detoteHrm-1in1′e,da nbadsefrdo mon HN-O6′Et oanHa-ly11sisin tdoigceattheedr twhaitthb tohteh cHou-6plainndg cHo-n6s′t,aanst.w Tehlel acosrHre-l1a1tioHn-s1 1fr′oamre Hin-6e tqou Hat-o1r1i’a, lapnods firtioomn ,Hw-6h′i ctoh Hes-t1a1b liinsdhiecdattehde thceant tbroatlhr iHng-6a asncdh Hai-r 6co′, nafis gwuerlal taiso nH.-1T1h aerer einla etiqvueatcoorniafilg puorsaittiioonn, owfhbiochth esdtiahbylidsrhoefdu rthane creinngtrsalw riansgd aest echrmaiirn ceodnfmigauirnaltyiobny. Tcohme praerlaintigveth ceocnofuigpulirnagticoonn sotfa nbtowthi thdithhyedreropfourrtaedn croinmgpso wunads sdaentdersmiminueldat emda1iHnlyN MbyR csopmecptraarionfgt rtahnes caonudpcliisnsgt ecroenoisstoamnte wrsi[t2h4 ]t.hTe hreepstoerrteeodc hceommipstoruynodfsH a-n1da nsdimHu-l2atwedas 1dHe tNerMmiRn esdpeacsttrraa nosf ctorannfisg uanradt iocins. sTtheriseoaisssoimgnemrse [n2t4]w. Tasheb astseerdeoocnhethmeisctoryu polfi nHg-1c oannsdt aHn-t2o wf acso mdeptoerumnidne1dw aist htraJns c=on3.f6igHurza,tiwonh.i cThhiis 1,2 s aclsosisgentmo etnhte wreapso bratesdedp oenn itchiter icnoounpeliEng( Jco′ n′s=tan4t. 3ofH czo)maps owuenldl a1s wthitehs Ji1m,2 2 ,3 u= l3a.t6e dH1zH, wNhMichR issp celoctsreu tmo t(hJ e= r4e.p0oHrtze)d[ 2p4e]n. iHciotrwineovneer ,Eth (eJ2′r,3e′ l=a t4iv.3e Hcozn) fiags uwraetlli oans othfeH s-i1m′ uanladteHd -12H′ wNaMs Rel uspciedcatrteudmt o(J b=e 4c.i0s dHuze) [t2o4t]h. He coowuepvleinr,g thcoe nrestlaatnivt eo fcoJn′fi′g=ur6a.t4ioHn zo,f wHh-1ic′ hanwda Hs -m2′o wreass iemluiclaidr atotedth teo cboeu cpisli dnugec toon th1 ,2 stae nctoufoprlitnhge csoimnsutlaantet dofc isJ1c′,2o′ n=fi 6g.u4 rHatzio, nw(hJ i=ch8 .w2 Hasz )m[2o4re]. sTihmeildairff teor etnhtec oconufipgluinragt icoonnssotafntht ofsoer ttwheo dsiimhyudlarotefdu rcains crionngfsigcuarnaatilosno b(Je =c 8o.n2fi Hrmz)e [d24b]y. TthheeN dOiffEerceonrrt ecloantifoingsu.rFatoironthse ofif rtshtodsieh tywdoro dfuihrayndrroinfugrNanO rEincgosr rcealnat iaolsnos braen cgoenffriormmebdo tbhyH th-1e1 NaOndEH co-6r′retolaHtio-2n,sw. Fhoilre tfhoer tfhiresto dthiheryddirhoyfudrraonfu rrianngr NinOg,Et hceorrereislaatiNonOsE rcaonrgree lfartoiomn bfrootmh HH--161t oanHd- 1H0-′6i′n tsot eHad-2o, fwHh-i2le. fTohre trheefo orteh,ethr edirhelyadtirvoefucorannfi griunrga,t itohneroef icso am NpOouEn cdor1rwelaatsioanss figronmed Has- 6sh too wHn-1i0n’ Finigstueraed3 o. f H-2. Therefore, the relative configuration of compound 1 was assigned as shown in Figure 3. FFiigguurree 33.. KKeeyy HHMMBBCC aanndd NNOOEE ccoorrrreellaattiioonnss ooff ccoommppoouunndd 11.. Mar. Drugs 2020, 18, 227 5 of 12 Table 1. 1H (600 MHz) and 13C (150 MHz) NMR data of 1 (CD3OD; δ in ppm). Position 13C 1H HMBC 1 89.2 (CH) 4.43 qd (6.4, 3.6) C-3, C-8, C-9 2 45.3 (CH) 2.99 qd (6.7, 3.6) C-10, C-3, C-8 3 140.0 (Cq) 4 55.6 (Cq) 5 199.5 (Cq) 6 73.1 (CH) 3.63 s C-4, C-5, C-7, C-8, C-3′, C-4′, C-5′, C-11′ 7 184.7 (Cq) 8 150.3 (Cq) 9 20.6 (CH3) 1.24 d (6.4) C-1, C-2 10 20.3 (CH3) 1.22 d (6.7) C-1, C-2, C-3 11 16.1 (CH3) 1.325 s C-3, C-4, C-5, C-6′, C-8 1′ 88.3 (CH) 4.37 p (6.4) C-3′, C-8′, C-10′ 2′ 47.1 (CH) 2.91 p (7.0) C-1′, C-3′, C-8′, C-9′ 3′ 139.2 (Cq) 4′ 55.1 (Cq) 5′ 199.0 (Cq) 6′ 71.9 (CH) 3.71 s C-3, C-4, C-5, C-11, C-4′, C-5′, C-7′, C-8′ 7′ 184.2 (Cq) 8′ 150.8 (Cq) 9′ 20.7 (CH3) 1.30 d (6.4) C-1′, C-2′ 10′ 19.3 (CH3) 1.36 d (7.0) C-1′, C-2′, C-3′ 11′ 14.5 (CH ) 1.332 s C-6, C-3′, C-4′, C-5′, C-8′3 For compounds 2–4, structure elucidation based on 1D and 2D NMR spectra as well as LC-HRMS, led to the known secondary metabolites penicitrinone A (2) [25], penicitrinone E (3) [26], and penicitrinol J (4) [26]. Those structures were additionally confirmed by comparison with the published NMR data [25,26]. 2.2. Bioactivity In order to gain insight into the biological activity of compounds 1–4, they were tested against a panel of eight different bacteria (Bacillus megaterium DSM32, Bacillus subtilis JH642, Bacillus subtilis DSM10, Micrococcus luteus ATCC 4698, Mycobacterium smegmatis ATCC607, Listeria monocytogenes DSM20600, Staphylococcus aureus ATCC25923, Escherichia coli K12), as well as against the yeast Candida albicans FH2173, and the mold fungus Aspergillus flavus ATCC9170. The results of the bioactivity assays are summarized in Table 2. Only compounds 2 and 4 revealed moderate activities against Gram-positive strains, while no effect was observed against Gram-negative bacteria and fungi. Table 2. Bioactivity of compounds 1–4. MIC (µg/mL) a Microorganisms Tested Penicitrinone G (1) Penicitrinone A (2) Penicitrinone E (3) Penicitrinol J (4) Bacillus megaterium DSM32 >64 >64 >64 16 Bacillus subtilis JH642 >64 >64 >64 16 Bacillus subtilis DSM10 >64 >64 >64 64 Micrococcus luteus ATCC4698 >64 >64 >64 >64 Mycobacterium smegmatis ATCC607 >64 32 >64 32 Listeria monocytogenes DSM20600 >64 >64 >64 >64 Staphylococcus aureus ATCC25923 >64 >64 >64 64 Escherichia coli K12 >64 >64 >64 >64 Candida albicans FH2173 >64 >64 >64 >64 Aspergillus flavus ATCC9170 >64 >64 >64 >64 a Activities are highlighted in bold. Mar. Drugs 2020, 18, 227 6 of 12 3. Discussion 3.1. Bioactivity As can be seen in Table 2, penicitrinol J (4) exhibited moderate activity against B. megaterium, B. subtilis JH642, and M. smegmatis (with corresponding MICs of 16, 16, and 32 µg/mL), whereas penicitrinone A (2) only showed weak activity against M. smegmatis. The activity against M. smegmatis was of the same magnitude for compounds 2 and 4 (MIC of 32 µg/mL, respectively). On the other hand, penicitrinone E (3) and the new compound 1 were inactive against the chosen test strains. Comparing the structures of compounds 2 and 3, the only difference is an additional carboxylic acid group in compound 3, which might imply that the carboxylic acid group slightly decreases the antibacterial activity. A likewise structural comparison of compounds 3 and 4 in relation to their bioactivities, suggests that phenolic systems (4) provide increased antibacterial potency in contrast to chinoid systems (3). In this case, the benefit of the aromatic system even seems to outweigh the effect of the carboxylic acid function. The increased activity of penicitrinol J (4) as opposed to penicitrinone E (3) was previously observed in a paper diffusion assay, but not quantified by determination of the respective MIC values [26]. The idea, that the antibacterial activity might be related to the presence of a phenolic system can be supported by the results of Yang et al. [27], who reported that penicitrinol A shows superior antibiotic activity to penicitrinone A (2) when tested against M. luteus and E. coli. Whether the difference in bioactivity of compounds 3 and 4 is to be attributed to structural factors or to their different redox properties still needs to be investigated. For the related compound citrinin, its redox properties seem to play an important role for its bioactivity, especially for its reported anti-fungal effect [28–30]. In contrast to previous reports, in the present study no activity could be detected for penicitrinone A (2) against M. luteus [27], E. coli [27] or B. megaterium [31].The lack of anti-fungal activity determined for compound 2 against C. albicans and A. flavus on the other hand, is in accordance with earlier findings [25]. 3.2. Biosynthesis Due to the interesting structure of the new compound 1, we were wondering what type of reactions are involved in its biosynthesis. To hypothesize a biosynthetic pathway for compound 1, first the proposed biosyntheses of penicitrinone and penicitrinol derivatives were analyzed [26,32–34]. In contrast to the biosynthesis of most citrinin derivatives, which include a Diels–Alder reaction, we propose a radical pathway for the formation of compound 1. This assumption is based on a similar mechanism reported for the formation of the structurally related compound dibefurin [35] (Figure 4). We assume that the key precursor for the formation of compound 1 is 2,3,4-trimethyl-5,6,7-trihydroxy-2,3-dihydrobenzofuran. Even though this substance itself is not known to date, it constitutes one of the monomers forming penicitol B [36]. The key intermediate might arise from the oxidation of 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran, a compound frequently found in extracts of P. citrinum species [25,26,37]. This substance in turn, could either be produced directly via the polyketide pathway, as shown in Figure 4, or might alternatively be a decomposition product of citrinin as proposed by Clark et al. [24]. Starting point of our hypothesis is the pentaketide I, which is C-methylated by a radical SAM enzyme and then yields after aldol condensation and keto-enol tautomerization the enzyme bound aromatic compound IV. Labelling experiments for the elucidation of the citrinin biosynthesis [33] seem to support our assumption of C-methylation of an unbranched pentaketide. Reductive release from the enzyme [33], yielding the aromatic aldehyde V is also suggested in analogy to the biosynthesis of citrinin. After reduction of the ketone to the alcohol, we speculate that the aldehyde might undergo a Dakine type oxidation, thus producing a phenol (VIII). Condensation of VIII affords the heterocyclic compound IX, which after oxidation furnishes the precursor X. Selective formation of a five-membered ring instead of a six-membered ring might either be ascribed to the fact that formation of five-membered rings is kinetically favoured over the formation of six-membered rings or alternatively an enzyme might promote the selectivity of the reaction. Another Mar. Drugs 2019, 17, x FOR PEER REVIEW 7 of 12 trihydroxy-2,3-dihydrobenzofuran. Even though this substance itself is not known to date, it constitutes one of the monomers forming penicitol B [36]. The key intermediate might arise from the oxidation of 2,3,4-trimethyl-5,7- dihydroxy-2,3-dihydrobenzofuran, a compound frequently found in extracts of P. citrinum species [25,26,37]. This substance in turn, could either be produced directly via the polyketide pathway, as shown in Figure 4, or might alternatively be a decomposition product of citrinin as proposed by Clark et al. [24]. Starting point of our hypothesis is the pentaketide I, which is C-methylated by a radical SAM enzyme and then yields after aldol condensation and keto-enol tautomerization the enzyme bound aromatic compound IV. Labelling experiments for the elucidation of the citrinin biosynthesis [33] seem to support our assumption of C- methylation of an unbranched pentaketide. Reductive release from the enzyme [33], yielding the aromatic aldehyde V is also suggested in analogy to the biosynthesis of citrinin. After reduction of the ketone to the alcohol, we speculate that the aldehyde might undergo a Dakine type oxidation, thus producing a phenol (VIII). Condensation of VIII affords the heterocyclic compound IX, which after oxidation furnishes the precursor X. Selective formation of a five-memberedM arri.nDgr uignss2t0e2a0d, 1o8,f2 2a7 six-membered ring might either be ascribed to the fact that formation of fiv7eo-f 12 membered rings is kinetically favoured over the formation of six-membered rings or alternatively an enzyme might promote the selectivity of the reaction. Another route for the biosynthesis of X could proceed by the same pathway outlirnoeudt effoorr tchiteribniions yn[t3h3e] siswoifthX cotuhled pcrroucceieadl byinttheermsaemdieatpe athbweianyg ou2tl,i4n-dedihfyodrrocixtyri-n5-inm[e3th3]ylw-6i-t(h3-the oxobutan-2-yl)cirsuopcihatlhianltaelrdmeheyddiaet.e Abe itnwgo2fo,4ld-d iDhyakdirno xtyy-p5e-m oextihdyalt-i6o-n(3 -oofx othbius taanr-o2m-yalt)iics odpihatldheahlayldee,h yfodlelo. wAetdw obfyo ld condensation Damkiignhtty pefoorxmid atXio. n oYfetth, is aarso mtahtiec ditawldoefohlydd e,Dfoalkloinw edtybpye conodxiednastaiotino n mwioguhltdf orlmeaXd . Yetot, asat he tetrahydroxylattwedo foalrdomDaatkici nctoymppeoouxnida, twioen wdeoeumld tlheiasd ptaothawteatyra hleysds rloikxeyllya.t eCdoamropmouantidc cXo mfipnoalulyn du,nwdeerdgeoeems ath is radical dimeripzatthiowna yprleoscseslisk ealsy .oCuotlminpeodu innd dXefitanial lliyn uFnigduerge o4e,s awriathd icthael dsiemqeureinzaceti oonf porxoicdeastsioans oauntdli nreduicntidoent ail reactions yieldiin gF itghue rieso4l,awteidth cothmepsoeuqnude n1c. e of oxidation and reduction reactions yielding the isolated compound 1. FFigiguurere4 4. . PPrrooppoosseedd bbiioossyynntthheettiicc ppaatthhwwaayy oof fcocommppouonudn d1. 1. 4. 4M.. aMteartiearlisalasn adndM Metehtohdosds 4.14..1S.p SopnognegCe oClolellcetcitoinon TeTnensp sopnognegsesw weerree ccoolllleecctteedd frforomm HHogoag IaslIasnldan wdatwerast einr sthien WthaekaWtoabki aNtoabtiionNaal tPioarnka ,l SPoaurtkh, ESaosut th EaSsut lSauwlaewsi,e Isni,dIonndeosinae (s5i°a2(85'2◦42.86′82"4S. 6a8n′d′S 1a2n3°d451'2136◦.6465"′E1)6 i.n66 A′′uEg)uinst A20u1g6u bsyt 2s0cu16bab dyisvcinugb aatd 2i0v imng daetp2th0 m de(pFtihgu(rFei g1u), raen1d) ,daoncudmdeonctuedm aesn WtedK-aPs1,W WKK--PP12,, WWKK--PP32,, WWKK-P-P53, ,WWKK-P-7P, 5W, WK-KP8-,P W7,KW-PK9-, PW8,KW-P1K1-,P 9, WKW-KP-1P11,2W, aKn-dP W12K, a-Pn2d0.W AKcc-oPr2d0i.nAg ctoc orredfeirnegnctoe [r3e8f]e,r tehnec sep[e3c8im], ethnes wspeercei mcaetengsowrizeerde cina ttehgeo froilzleodwiinngt he folgloenweirnag: Cgleantherriaa :spC.l WathKr-iaP1s;p S.uWbeKre-aP s1p;. SWuKbe-rPe2a; sSpu.bWereKa -sPp2. ;WSKub-Per9e;a Nsepo.peWtroKs-iaP s9p; .N WeoKp-ePtr3o; sCiainsapc.hyWreKlla-P 3; Cinspac. hWyrKel-lPa5s; pC.arWterKio-sPp5o;ngCiaar stepr.i oWspKo-nPg8ia; Hspy.rtiWos Ksp-P. 8W; HK-yPr1ti1o;s asnpd. AWapKto-Ps 1s1p;. aWnKd-PA1a2p.t oOsnslpy .3W–5 Kcm-P 12. material were taken from each specimen. All samples were photo documented underwater and Only 3–5 cm material were taken from each specimen. All samples were photo documented underwater and labelled before storage. Samples were placed in a zip-lock plastic bag, temporarily stored in a cool box and directly processed in the laboratory. 4.2. Fungal Isolation and Purification The isolation of sponge-associated fungi was done according to Kjer’s protocol [39]. Sponges were initially sprayed with sterile natural seawater, cut into three pieces with an approximate size of 1 cm2 and placed into malt extract agar (MEA) (Himedia, Mumbai, India) medium (30 g malt extract, 5 g mycological peptone, 15 g agar, and 1000 mL sterile natural seawater, hereinafter referred to as marine MEA). The agar plates were incubated at room temperature (25 ◦C) for three days. The initial selection of fungal colonies was done based on phenotype, e.g., colony morphology and color [40]. In total, 20 fungal colonies were isolated and propagated until axenic cultures were obtained from ten sponges. In an initial screening, using the agar plug method, five strains were active and strain WK-P9 was selected for further investigation due to its prominent activity. Mar. Drugs 2020, 18, 227 8 of 12 4.3. Molecular Identification of the Fungus DNA amplification was performed using the Toyobo KOD FX Neo kit (Toyobo, Osaka, Japan). Fungus WK-P9 was grown in marine MEA for 3 days; a loop of mycelia was transferred into 2 µL PCR grade water, which served as template for colony PCR. The PCR mixture was set up in a total volume of 50 µL as follows: PCR grade water 9 µL, 2× PCR buffer for KOD FX Neo 25 µL, 2mM dNTPs 10 µL D, primer ITS1 (5’-tccgtaggtgaacctgcgg-3’, 10 pmol/µL) 1.5 µL, primer ITS4 (5’-tcctccgcttattgatatgc-3’, 10 pmol/µL) 1.5 µL, DNA template 2 µL (from mycelia), KOD FX Neo (1.0 U/µL) 1 µL. On a T100™ Thermal Cycler from Bio-Rad (Feldkirchen, Germany), the PCR program was run as follows: Denaturation initially at 95 ◦C for 3 min, followed by 35 cycles (denaturation at 95 ◦C for 3 min, annealing at 55 ◦C for 45 s, and extension at 72 ◦C for 1 min), then 72 ◦C extension for 7 min and cooling to 16 ◦C. The PCR product was Sanger-sequenced (1st BASE DNA sequencing services). Then, MEGA X was used to generate an alignment and the phylogenetic tree using the sequence data obtained. The phylogenetic tree was constructed using the Maximum likelihood and Neighbor-Joining analysis, with 1000 replications of bootstrap value (Figure S8). The sequence data was submitted to GenBank (Acc. Number: LC371661.1). 4.4. Isolation and Structure Elucidation Three pieces of P. citrinum WK-P9 (each 1 × 1 cm2), which nearly covered the whole surface of a Petri dish, were inoculated onto malt extract broth (Himedia, Mumbai, India) media diluted by sterilized natural seawater (12 L) under clean-bench conditions, and cultivated for 12 days at 24 ◦C. The crude extract of P. citrinum WK-P9 (4.0 g) was collected after soaking the medium with ethyl acetate (EtOAc) using the ratio 1:3. The purification was initially performed by Silica (Wako, Japan) vacuum liquid chromatography (VLC) using a gradient composed of three different solvent systems-n-hexan:EtOAc (7:3); DCM:MeOH (85:15); EtOAc:MeOH (85:15), yielding five fractions. By activity-guided isolation, three active fractions were further purified by Sephadex LH-20 (GE Healthcare Europe GmbH, Freiburg, Germany), reverse phase silica C-18 (Interchim Puriflash 4125 Chromatography system with a Puriflash C18-HP 30µm F0080 Flash column), yielding 12 fractions. The final purification was conducted by HPLC (column EC 250/4.6 Nucleodur C18 Gravity-SB, 5µm) at a flowrate of 1 mL/min. The respective HPLC solvent gradients applied for the purification of the four compounds described in this paper are as follows: Compound 1 was obtained using a gradient of 55–60% MeOH/H2O within 25 min. Compound 2 was purified using a gradient of 40–46.5% Acetonitrile/H2O + 0.01% TFA within 15 min. Compound 3 was isolated using a gradient of 45–55% Acetonitrile/H2O + 0.01% TFA within 15 min. Compound 4 was obtained using isocratic elution of 87% MeOH/H2O + 0.01% TFA. Penicitrinone G (1): White amorphous solid; [α]24D = 16.25 (c 0.03, MeOH): 1H (600 HMz) and 13C (150 MHz) NMR (CD3OD), see Table 1; LC-HRESIMS m/z 385.1653 [M + H]+ and 407.1489 [M + Na]+ (calculated mass for C22H25O6: m/z = 385.1646). 4.5. Antibacterial Susceptibility Tests The antimicrobial activity of the crude extract and the following sub-fractions was conducted using the agar diffusion method [41]. The respective test bacteria (Escherichia coli K12, Bacillus megaterium DSM32, Bacillus subtilis JH642, Micrococcus luteus ATCC4698) were spread on Luria Bertani (LB) agar plates (10 g peptone, 5 g yeast extract, 5 g NaCl, 15 g agar, mixed with 1 L distilled water). For sample preparation, 15 µL of each crude extract (10 mg/mL dissolved in methanol) or fraction were added to a paper disk. Methanol was used as the negative control and carbenicillin (5 µL of a 50 mg/mL stock solution) (Carl Roth GmbH + Co., Karlsruhe, Germany) was used as positive control. The dried paper disks were subsequently positioned on the agar plate and incubated at 30 ◦C overnight. The diameter of the resulting inhibition zone was determined. Determination of the minimum inhibitory concentrations (MIC) of purified compounds 1–4 was carried out by micro broth dilution assays in 96 well plates. All compounds were dissolved in Mar. Drugs 2020, 18, 227 9 of 12 dimethyl sulfoxide (DMSO, Carl Roth GmbH + Co., Karlsruhe, Germany) and tested in triplicate. For B. subtilis DSM10 and S. aureus ATCC25923, an overnight culture (37 ◦C, 180 rpm) was diluted to 5 × 105 cells/mL in cation adjusted Mueller Hinton II medium (Becton Dickinson, Sparks, NV, USA). L. monocytogenes DSM20600 was incubated for 2 days before the assay inoculum was adjusted using the same medium and growth conditions (Mueller Hinton II medium). As positive controls, dilution series of rifampicin, tetracycline and gentamycin (all Sigma Aldrich, St. Louis, MS, USA) were prepared (64–0.03 µg/mL). Cell suspensions without test sample or antibiotic control were used as negative controls. After incubation (18 h and 48 h for L. monocytogenes, 37 ◦C, 180 rpm, 80% rH) cell growth was assessed by turbidity measurement with a microplate spectrophotometer at 600 nm (LUMIstar®Omega BMG Labtech, Ortenberg, Germany). The pre culture of M. smegmatis ATCC607 was incubated in Brain-Heart Infusion broth (Becton Dickinson) supplemented with Tween 80 (1.0% (v/v)) for 48 h at 37 ◦C and 180 rpm before the cell concentration was adjusted in cation adjusted Mueller Hinton II medium. Isoniazid (Sigma-Aldrich) was used instead of gentamycin as a third positive control. Cell viability was evaluated after 48 h (37 ◦C, 180 rpm, 80% rH) via ATP quantification (BacTiter-Glo™, Promega, Madison, WI, USA) according to the manufacturer’s instructions. C. albicans FH2173 was incubated for 48 h at 28 ◦C and 180 rpm before the pre culture was diluted to 1 × 106 cells/mL in cation adjusted Mueller Hinton II medium. For A. flavus ATCC9170, a previously prepared spore solution was used to prepare the assay inoculum of 1 × 105 spores/mL. Yeast and mold assays were incubated for 48 h at 37 ◦C, 180 rpm and 80% rH. For both, tebuconazole (Cayman Chemical Company, Ann Arbor, MI, USA), amphotericin B and nystatin (both Sigma Aldrich) were used as positive control (64–0.03 µg/mL). The readout was carried out by ATP quantification. 5. Conclusions In summary, by applying bioassay-guided fractionation, four citrinin derivatives were obtained from the sponge-associated fungus P. citrinum WK-P9. Among those substances, the new derivative 1 was characterized for the first time, revealing a connection of monomers which has so far been unprecedented for citrinin derivatives. To provide an explanation for the formation of this new derivative, a biosynthetic hypothesis was proposed. Furthermore, all isolated compounds were screened for bioactivity. In this respect, penicitrinol J (4) showed moderate antimicrobial activity against B. subtilis JH642, B. megaterium DSM32, and M. smegmatis ATCC607. Even though P. citrinum is a well-investigated fungal species, such strains still have an inherent high possibility to deliver new specialized metabolites, as exemplified by the isolation of the new compound 1. New strains in combination with variation in culture conditions, e.g., using the one strain many compounds (OSMAC) approach, thus represent a promising bioresource for natural product discovery. Supplementary Materials: The following are available online at http://www.mdpi.com/1660-3397/18/4/227/s1, Figures S1–S6: NMR data of compound 1; Figure S7: LC-HRESIMS of compound 1; Figure S8 phylogenetic tree of fungus Penicillium citrinum. Author Contributions: O.K.R., A.S. (Agus Sabdono) and A.T. created the idea and planned the research. T.F.S., Y.L., and U.M. designed the experiments and analyzed the data. A.S. (Aninditia Sabdaningsih), Y.L., J.H., R., L.W., O.C., H.N., M.T.S. and M.M. performed the experiments and analyzed the data. A.S. (Aninditia Sabdaningsih), Y.L., U.M. and T.F.S. wrote the manuscript, which was edited by all authors. All authors have read and agreed to the published version of the manuscript. Funding: This research was funded by the PMDSU (Program of Master Degree Leading to Doctoral Degree for Excellent Graduates) Scholarship from Kementerian Riset, Teknologi dan Pendidikan Tinggi Republik Indonesia with contract number 315-06/UN7.5.1/PP/2017. A.S.N. performed work at the Justus-Liebig-University of Giessen through the Enhancing International Publication Program from Kementerian Riset, Teknologi dan Pendidikan Tinggi Republik Indonesia with contract number 1406.36/D3/PG/2018. The Federal Ministry of Education and Research (BMBF) supported the work in the lab of T.F.S. with the grant 16GW0117K. Acknowledgments: The authors thank Jean-Marie Pohl for fruitful discussions and valuable advice concerning the biosynthesis, as well as Heike Hausmann (Justus-Liebig-University Giessen) for measuring the NMR spectra. Conflicts of Interest: The authors declare no conflict of interest. Mar. Drugs 2020, 18, 227 10 of 12 References 1. Hu, G.P.; Yuan, J.; Sun, L.; She, Z.G.; Wu, J.H.; Lan, X.J.; Zhu, X.; Lin, Y.C.; Chen, S.P. Statistical Research on Marine Natural Products Based on Data Obtained between 1985 and 2008. Mar. Drugs 2011, 9, 514–525. [CrossRef] [PubMed] 2. Abad, M.J.; Bedoya, L.M.; Bermejo, P. Marine compounds and their antimicrobial activities. Fortamex 2011, 51, 1293–1306. 3. Dyshlovoy, S.A.; Fedorov, S.N.; Shubina, L.K.; Kuzmich, A.S.; Bokemeyer, C.; Keller-Von Amsberg, G.; Honecker, F. 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