Izar, BenjaminBenjaminIzarMraheil, Mobarak AbuMobarak AbuMraheilHain, TorstenTorstenHain2022-11-182011-09-022022-11-182011http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-83356https://jlupub.ub.uni-giessen.de/handle/jlupub/9611http://dx.doi.org/10.22029/jlupub-8999Bacterial regulatory non-coding RNAs control numerous mRNA targets that direct a plethora of biological processes, such as the adaption to environmental changes, growth and virulence. Recently developed high-throughput techniques, such as genomic tiling arrays and RNA-Seq have allowed investigating prokaryotic cis- and trans-acting regulatory RNAs, including sRNAs, asRNAs, untranslated regions (UTR) and riboswitches. As a result, we obtained a more comprehensive view on the complexity and plasticity of the prokaryotic genome biology. Listeria monocytogenes was utilized as a model system for intracellular pathogenic bacteria in several studies, which revealed the presence of about 180 regulatory RNAs in the listerial genome. A regulatory role of non-coding RNAs in survival, virulence and adaptation mechanisms of L. monocytogenes was confirmed in subsequent experiments, thus, providing insight into a multifaceted modulatory function of RNA/mRNA interference. In this review, we discuss the identification of regulatory RNAs by high-throughput techniques and in their functional role in L. monocytogenes.enNamensnennung 3.0 Internationalregulatory RNAListeria monocytogenesRNA interferencewhole genome tiling arraysinfectionddc:610