Kämpfer, PeterPeterKämpferSchäfer, JennyJennySchäferLodders, NadineNadineLoddersBusse, Hans-JürgenHans-JürgenBusse2023-06-122012-02-152023-06-122009http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-86135https://jlupub.ub.uni-giessen.de/handle/jlupub/17132http://dx.doi.org/10.22029/jlupub-16510A Gram-stain-positive, rod-shaped, non-endospore-forming, orange-pigmented (coloured) actinobacterium (01-Je-003T) was isolated from the wall of an indoor environment primarily colonized with moulds. On the basis of 16S rRNA gene sequence similarity studies, strain 01-Je-003T was shown to belong to the genus Brevibacterium and was most similar to the type strains of Brevibacterium picturae (98.8% similarity), Brevibacterium marinum (97.3%) and Brevibacterium aurantiacum (97.2 %). Chemotaxonomic data [predominant quinone menaquinone MK-8(H2); polar lipid profile consisting of major compounds diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid meso-diaminopimelic acid; polyamine pattern showing major compounds putrescine and cadaverine; major fatty acids anteiso-C15:0 and anteiso-C17:0] supported the affiliation of strain 01-Je-003T to the genus Brevibacterium. The results of DNA DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 01-Je-003T from the two most closely related species, B. picturae and B. marinum. Strain 01-Je-003T therefore represents a novel species, for which the name Brevibacterium sandarakinum sp. nov. is proposed, with the type strain 01-Je-003T (5DSM 22082T 5CCM 7649T).enIn CopyrightBrevibacterium sandarakinum sp. nov.Actinobacteriaddc:570