Bommer, TheaTheaBommerKnierim, MariaMariaKnierimUnsöld, JuliaJuliaUnsöldRiedl, DominicDominicRiedlStengel, LauraLauraStengelPaulus, MichaelMichaelPaulusKörtl, ThomasThomasKörtlLiaw, NormanNormanLiawMaier, Lars S.Lars S.MaierStreckfuss-Bömeke, KatrinKatrinStreckfuss-BömekeSossalla, SamuelSamuelSossallaPabel, SteffenSteffenPabel2024-12-182024-12-182024https://jlupub.ub.uni-giessen.de/handle/jlupub/20090https://doi.org/10.22029/jlupub-19445The effects and mechanisms of cardiac arrhythmias are still incompletely understood and an important subject of cardiovascular research. A major difficulty for investigating arrhythmias is the lack of appropriate human models. Here, we present a protocol for a translational simulation of different types of arrhythmias using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and electric cell culture pacing. The protocol comprises the handling of ventricular and atrial hiPSC-CM before and during in vitro arrhythmia simulation and possible arrhythmia simulation protocols mimicking clinical arrhythmias like atrial fibrillation. Isolated or confluent hiPSC-CM can be used for the simulation. In vitro arrhythmia simulation did not impair cell viability of hiPSC-CM and could reproduce arrhythmia associated phenotypes of patients. The use of hiPSC-CM enables patient-specific studies of arrhythmias, genetic interventions, or drug-screening. Thus, the in vitro arrhythmia simulation protocol may offer a versatile tool for translational studies on the mechanisms and treatment options of cardiac arrhythmias.enNamensnennung 4.0 Internationalddc:610