Arneth, Borros M.Borros M.Arneth2022-11-182020-08-182022-11-182018http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-153971https://jlupub.ub.uni-giessen.de/handle/jlupub/9574http://dx.doi.org/10.22029/jlupub-8962This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the expression of extracellular activation markers on and intracellular cytokines in T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with beta-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extra-cellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases.enNamensnennung 4.0 Internationalregulatory T cellsCD4+ T helper cellslymphocytesCD8+ cytotoxic T cellsinterferon-gammaddc:610