Schröck, CarmenCarmenSchröckEydt, CarinaCarinaEydtGeburek, FlorianFlorianGeburekKaiser, LenaLenaKaiserPäbst, FelicitasFelicitasPäbstBurk, JaninaJaninaBurkPfarrer, ChristianeChristianePfarrerStaszyk, CarstenCarstenStaszyk2022-11-182019-08-012022-11-182017http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-147815https://jlupub.ub.uni-giessen.de/handle/jlupub/9493http://dx.doi.org/10.22029/jlupub-8881Abstract Allogeneic equine multipotent mesenchymal stromal cells (eMSCs) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSCs might be the bone marrow from euthanized horses. The purpose of this study was to compare in vitro characteristics of equine bone marrow derived eMSC (eBM-MSCs) from euthanized horses (eut-MSCs) and from narcotized horses (nar-MSCs). Eut-MSCs and nar-MSCs showed typical eMSC marker profiles (positive: CD44, CD90; negative: CD11a/CD18 and MHCII) and possessed tri-lineage differentiation characteristics. Although CD105 and MHCI expression varied, no differences were detected between eut-MSCs and nar-MSCs. Proliferation characteristics did not differ between eut-MSCs and nar-MSCs, but age dependent decrease in proliferation and increase in MHCI expression was detected. These results suggest the possible use of eut-MSCs for therapeutic applications and production of commercial available eBM-MSC products.enNamensnennung, Nicht kommerziell, keine Bearbeitung 4.0 Internationalequine stem cellsflow cytometryhorseproliferation assayregenerative therapyddc:630