Reutzel, MartinaMartinaReutzelGrewal, RekhaRekhaGrewalSilaidos, CarminaCarminaSilaidosZotzel, JensJensZotzelMarx, StefanStefanMarxTretzel, JoachimJoachimTretzelEckert, Gunter P.Gunter P.Eckert2022-11-182020-06-032022-11-182018http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-151857https://jlupub.ub.uni-giessen.de/handle/jlupub/9517http://dx.doi.org/10.22029/jlupub-8905Aging represents a major risk factor for developing neurodegenerative diseases such as Alzheimer´s disease (AD). As components of the Mediterranean diet, olive polyphenols may play a crucial role in the prevention of AD. Since mitochondrial dysfunction acts as a final pathway in both brain aging and AD, respectively, the effects of a mixture of highly purified olive secoiridoids were tested on cognition and ATP levels in a commonly used mouse model for brain aging. Over 6 months, female NMRI mice (12 months of age) were fed with a blend containing highly purified olive secoiridoids (POS) including oleuropein, hydroxytyrosol and oleurosid standardized for 50 mg oleuropein/kg diet (equivalent to 13.75 mg POS/kg b.w.) or the study diet without POS as control. Mice aged 3 months served as young controls. Behavioral tests showed deficits in cognition in aged mice. Levels of ATP and mRNA levels of NADH-reductase, cytochrome-c-oxidase, and citrate synthase were significantly reduced in the brains of aged mice indicating mitochondrial dysfunction. Moreover, gene expression of Sirt1, CREB, Gap43, and GPx-1 was significantly reduced in the brain tissue of aged mice. POS-fed mice showed improved spatial working memory. Furthermore, POS restored brain ATP levels in aged mice which were significantly increased. Our results show that a diet rich in purified olive polyphenols has positive long-term effects on cognition and energy metabolism in the brain of aged mice.enNamensnennung 4.0 Internationalddc:610