Maniar, AmudhanAmudhanManiar2023-03-032007-11-232023-03-032007978-3-8359-5211-0http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-50978https://jlupub.ub.uni-giessen.de/handle/jlupub/10691http://dx.doi.org/10.22029/jlupub-10074This study sheds light on the characterisation of CD177 and identification of CD177s counter receptor on endothelium. We could demonstrate that PECAM-1 on endothelial cells functions directly as the counter receptor for CD177 by following observations; 1. Our recombinant CD177-Fc fusion protein, which was produced in insect cells, reacted with 130 KDa molecules on endothelium. 2. Screening with different mabs identified the 130 KDa protein as endothelial PECAM-1. 3. Biochemical and immunological studies using recombinant CD177-Fc fusion protein confirmed that endothelial PECAM-1 functions directly as the counter-receptor for CD177. 4. Functional studies in vitro demonstrated that purified CD177 protein inhibits CD177 mediated cell adhesion to immobilized PECAM-1 protein as well as neutrophil migration through endothelial cells. These results indicate that CD177 can act as cell adhesion molecule involving neutrophil-endothelial interaction. This observation may provide a new concept in understanding the multi-step process of transendothelial migration.In the future, this concept should be studied in different in vitro as well as in vivo model in more detail.enIn Copyrightddc:570