Kuhn, AnjaAnjaKuhnWeiler, KristinaKristinaWeilerGibbs, GrahamGrahamGibbsPrechtl, GottfriedGottfriedPrechtlBauer, NataliNataliBauerMoritz, AndreasAndreasMoritz2023-12-072023-12-072023https://jlupub.ub.uni-giessen.de/handle/jlupub/18771http://dx.doi.org/10.22029/jlupub-18135Background: A novel method using a moving threshold (r-PLTmt) to determine canine r-PLTs (reticulated platelets) has been introduced for ADVIA 2120 software v6.11.7. Objectives: We aimed to evaluate absolute (ar-PLTmt) and percent (%r-PLTmt) prior to and after visual inspection of scattergrams (ar-PLTmtv, %rPLTmtv) compared with flow cytometry (flow) and to determine reference intervals (RIs) in 120 dogs. Methods: For method comparison, 42 blood samples of healthy and thrombocytopenic dogs were included. Calculation of Spearman's rho, Bland–Altman, and Passing-Bablok analysis was performed. Coefficients of variation (CVs) were determined for three concentration levels. Results: Moderate correlations between %r-PLTmt and %r-PLTmtv (rs 0.75–0.76) were seen compared with flow cytometry. The CV for medium %r-PLTs counts assessed with flow cytometry was 12.9%. Comparable CVs were obtained for ar-PLTmt (14.4%) and %r-PLTmt (15.7%), and ar-PLTmtv and %r-PLTmtv (10.9% and 12.9%, respectively). At low and high concentration levels, CVs for % and absolute r-PLTmt/rPLTmtv ranged between 23%–30% and 15%–20%. In patients with microcytic hypochromic erythrocytes, CVs for ar-PLTmt and %r-PLTmt were 36%–66%. Visual inspection of scattergrams resulted in a marked decrease in CV ranging between 15% and 20%. A proportional bias of 10.8% between %r-PLTmt and flow cytometry became lower (9.7%) after visual validation of scattergrams. Passing-Bablok analysis showed proportional and constant error. RIs for r-PLTmt and r-PLTmtv were 0.2%–3.8% and 0.6–10.2 × 109/L and 0.3%–4.5% and 1.1–10.3 × 109/L, respectively. Median values for %r-PLTmtv were higher in young adults (≤2 years) than in older dogs (P = 0.03). Conclusions: r-PLTmt and r-PLTmtv were moderately correlated with flow cytometry. Visual inspection of scattergrams is recommended.enNamensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 Internationalddc:630