Siebers, KathrinKathrinSiebersFink, BijanBijanFinkZakrzewicz, AnnaAnnaZakrzewiczAgne, AlisaAlisaAgneRichter, KatrinKatrinRichterKonzok, SebastianSebastianKonzokHecker, AndreasAndreasHeckerZukunft, SvenSvenZukunftKüllmar, MiraMiraKüllmarKlein, JochenJochenKleinMcIntosh, J. MichaelJ. MichaelMcIntoshTimm, ThomasThomasTimmSewald, KatherinaKatherinaSewaldPadberg, WinfriedWinfriedPadbergAggarwal, NupurNupurAggarwalChamulitrat, WaleeWaleeChamulitratSantoso, SentotSentotSantosoXia, WendyWendyXiaJanciauskiene, SabinaSabinaJanciauskieneGrau, VeronikaVeronikaGrau2022-11-182019-05-202022-11-182018http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-146306https://jlupub.ub.uni-giessen.de/handle/jlupub/9465http://dx.doi.org/10.22029/jlupub-8853While interleukin (IL)-1beta is a potent pro-inflammatory cytokine involved in host defense, high levels can cause life-threatening sterile inflammation including systemic inflammatory response syndrome. Hence, the control of IL-1beta secretion is of outstanding biomedical importance. In response to a first inflammatory stimulus such as lipopolysaccharide, pro-IL-1β is synthesized as a cytoplasmic inactive pro-form. Extracellular ATP originating from injured cells is a prototypical second signal for inflammasome-dependent maturation and release of IL-1beta. The human anti-protease alpha-1 antitrypsin and IL-1beta regulate each other via mechanisms that are only partially understood. Here, we demonstrate that physiological concentrations of alpha-1 antitrypsin efficiently inhibit ATP-induced release of IL-1beta from primary human blood mononuclear cells, monocytic U937 cells and rat lung tissue, whereas ATP-independent IL-1beta release is not impaired. Both, native and oxidized alpha-1 antitrypsin are active, suggesting that the inhibition of IL-1beta release is independent of the anti-elastase activity of alpha-1 antitrypsin. Signaling of alpha-1 antitrypsin in monocytic cells involves the lipid scavenger receptor CD36, calcium-independent phospholipase A2β and the release of a small soluble mediator. This mediator leads to the activation of nicotinic acetylcholine receptors, which efficiently inhibit ATP-induced P2X7 receptor activation and inflammasome assembly. We suggest that alpha-1 antitrypsin controls ATP-induced IL-1beta release from human mononuclear blood cells by a novel triple-membrane-passing signaling pathway. This pathway may have clinical implications for the prevention of sterile pulmonary and systemic inflammation.enNamensnennung 4.0 InternationalCD36CHRNA7CHRNA9CHRNA10inflammasomeddc:610