Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes

dc.contributor.authorVöllner, Frauke
dc.contributor.authorAli, Jawahir
dc.contributor.authorKurrle, Nina
dc.contributor.authorExner, Yvonne
dc.contributor.authorEming, Rüdiger
dc.contributor.authorHertl, Michael
dc.contributor.authorBanning, Antje
dc.contributor.authorTikkanen, Ritva
dc.date.accessioned2022-11-18T09:51:32Z
dc.date.available2017-02-03T09:03:45Z
dc.date.available2022-11-18T09:51:32Z
dc.date.issued2016
dc.description.abstractDesmosomes are adhesion plaques that mediate cell-cell adhesion in many tissues, including the epidermis, and generate mechanical resistance to tissues. The extracellular domains of desmosomal cadherin proteins, desmogleins and desmocollins, are required for the interaction with cadherins of the neighbouring cells, whereas their cytoplasmic tails associate with cytoplasmic proteins which mediate connection to intermediate filaments. Disruption of desmosomal adhesion by mutations, autoantibodies or bacterial toxins results in severe human disorders of e.g. the skin and the heart. Despite the vital role of desmosomes in various tissues, the details of their molecular assembly are not clear. We here show that the two members of the flotillin protein family directly interact with the cytoplasmic tails of desmogleins. Depletion of flotillins in human keratinocytes results in weakened desmosomal adhesion and reduced expression of desmoglein-3, most likely due to a reduction in the desmosomal pool due to increased turnover. In the absence of flotillins, desmoglein-3 shows an altered localisation pattern in the cell-cell junctions of keratinocytes, which is highly similar to the localisation observed upon treatment with pemphigus vulgaris autoantibodies. Thus, our data show that flotillins, which have previously been connected to the classical cadherins, are also of importance for the desmosomal cell adhesion.en
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-124639
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9248
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8636
dc.language.isoende_DE
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddcddc:610de_DE
dc.titleLoss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytesen
dc.typearticlede_DE
local.affiliationFB 11 - Medizinde_DE
local.opus.fachgebietMedizinde_DE
local.opus.id12463
local.opus.instituteInstitute of Biochemistryde_DE
local.source.freetextScientific Reports 6:28820de_DE
local.source.urihttps://doi.org/10.1038/srep28820

Dateien

Originalbündel
Gerade angezeigt 1 - 1 von 1
Lade...
Vorschaubild
Name:
10.1038_srep28820.pdf
Größe:
3.45 MB
Format:
Adobe Portable Document Format