CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs
dc.contributor.author | Schneider, Tim | |
dc.contributor.author | Hung, Lee-Hsueh | |
dc.contributor.author | Schreiner, Silke | |
dc.contributor.author | Starke, Stefan | |
dc.contributor.author | Eckhof, Heinrich | |
dc.contributor.author | Rossbach, Oliver | |
dc.contributor.author | Reich, Stefan | |
dc.contributor.author | Medenbach, Jan | |
dc.contributor.author | Bindereif, Albrecht | |
dc.date.accessioned | 2022-11-18T09:51:33Z | |
dc.date.available | 2017-02-03T09:06:32Z | |
dc.date.available | 2022-11-18T09:51:33Z | |
dc.date.issued | 2016 | |
dc.description.abstract | Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-co-immunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component. | en |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-124646 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9249 | |
dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8637 | |
dc.language.iso | en | de_DE |
dc.rights | Namensnennung 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.ddc | ddc:540 | de_DE |
dc.title | CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs | en |
dc.type | article | de_DE |
local.affiliation | FB 08 - Biologie und Chemie | de_DE |
local.opus.fachgebiet | Biochemie (FB 08) | de_DE |
local.opus.id | 12464 | |
local.opus.institute | Institute of Biochemistry | de_DE |
local.source.freetext | Scientific Reports 6:31313 | de_DE |
local.source.uri | https://doi.org/10.1038/srep31313 |
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