Highly Conserved Testicular Localization of Claudin-11 in Normal and Impaired Spermatogenesis
dc.contributor.author | Stammler, Angelika | |
dc.contributor.author | Lüftner, Benjamin Udo | |
dc.contributor.author | Kliesch, Sabine | |
dc.contributor.author | Weidner, Wolfgang | |
dc.contributor.author | Bergmann, Martin | |
dc.contributor.author | Middendorff, Ralf | |
dc.contributor.author | Konrad, Lutz | |
dc.date.accessioned | 2022-11-18T09:51:34Z | |
dc.date.available | 2017-02-03T09:40:34Z | |
dc.date.available | 2022-11-18T09:51:34Z | |
dc.date.issued | 2016 | |
dc.description.abstract | In this study we tested expression of tight junction proteins in human, mouse and rat and analyzed the localization of claudin-11 in testis of patients with normal and impaired spermatogenesis. Recent concepts generated in mice suggest that the stage-specifically expressed claudin-3 acts as a basal barrier, sealing the seminiferous epithelium during migration of spermatocytes. Corresponding mechanisms have never been demonstrated in humans. Testicular biopsies (n = 103) from five distinct groups were analyzed: normal spermatogenesis (NSP, n = 28), hypospermatogenesis (Hyp, n = 24), maturation arrest at the level of primary spermatocytes (MA, n = 24), Sertoli cell only syndrome (SCO, n = 19), and spermatogonial arrest (SGA, n = 8). Protein expression of claudin-3, -11 and occludin was analyzed. Human, mice and rat testis robustly express claudin-11 protein. Occludin was detected in mouse and rat and claudin-3 was found only in mice. Thus, we selected claudin-11 for further analysis of localization. In NSP, claudin-11 is located at Sertoli-Sertoli junctions and in Sertoli cell contacts towards spermatogonia. Typically, claudin-11 patches do not reach the basal membrane, unless flanked by the Sertoli cell body or patches between two Sertoli cell bodies. The amount of basal claudin-11 patches was found to be increased in impaired spermatogenesis. Only claudin-11 is expressed in all three species examined. The claudin-11 pattern is robust in man with impaired spermatogenesis, but the proportion of localization is altered in SCO and MA. We conclude that claudin-11 might represent the essential component of the BTB in human. | en |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-124671 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9252 | |
dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8640 | |
dc.language.iso | en | de_DE |
dc.rights | Namensnennung 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.ddc | ddc:610 | de_DE |
dc.title | Highly Conserved Testicular Localization of Claudin-11 in Normal and Impaired Spermatogenesis | en |
dc.type | article | de_DE |
local.affiliation | FB 11 - Medizin | de_DE |
local.opus.fachgebiet | Medizin | de_DE |
local.opus.id | 12467 | |
local.opus.institute | Institute of Anatomy and Cell Biology | de_DE |
local.source.freetext | PLoS One 11(8):e0160349 | de_DE |
local.source.uri | https://doi.org/10.1371/journal.pone.0160349 |
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