Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation

dc.contributor.authorFietz, Daniela
dc.contributor.authorMarkmann, M.
dc.contributor.authorLang, D.
dc.contributor.authorKonrad, L.
dc.contributor.authorGeyer, J.
dc.contributor.authorKliesch, S.
dc.contributor.authorChakraborty, Trinad
dc.contributor.authorHossain, Hamid
dc.contributor.authorBergmann, M.
dc.date.accessioned2022-11-18T09:51:21Z
dc.date.available2016-11-07T10:46:29Z
dc.date.available2022-11-18T09:51:21Z
dc.date.issued2015
dc.description.abstractBackground: Androgens play an important role for the development of male fertility and gained interest as growth and survival factors for certain types of cancer. Androgens act via the androgen receptor (AR/Ar), which is involved in various cell biological processes such as sex differentiation. To study the functional mechanisms of androgen action, cell culture systems and AR-transfected cell lines are needed. Transfection of AR into cell lines and subsequent gene expression analysis after androgen treatment is well established to investigate the molecular biology of target cells. However, it remains unclear how the transfection with AR itself can modulate the gene expression even without androgen stimulation. Therefore, we transfected Ar-deficient rat Sertoli cells 93RS2 by electroporation using a full length human AR. Results: Transfection success was confirmed by Western Blotting, immunofluorescence and RT-PCR. AR transfection-related gene expression alterations were detected with microarray-based genome-wide expression profiling of transfected and non-transfected 93RS2 cells without androgen stimulation. Microarray analysis revealed 672 differentially regulated genes with 200 up- and 472 down-regulated genes. These genes could be assigned to four major biological categories (development, hormone response, immune response and metabolism). Microarray results were confirmed by quantitative RT-PCR analysis for 22 candidate genes. Conclusion: We conclude from our data, that the transfection of Ar-deficient Sertoli cells with AR has a measurable effect on gene expression even without androgen stimulation and cause Sertoli cell damage. Studies using AR-transfected cells, subsequently stimulated, should consider alterations in AR-dependent gene expression as off-target effects of the AR transfection itself.en
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-123289
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9224
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8612
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subjecttransfectionen
dc.subjectgene expression analysisen
dc.subjectandrogen receptoren
dc.subjectsertoli cellsen
dc.subject.ddcddc:630de_DE
dc.titleTransfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulationen
dc.typearticlede_DE
local.affiliationFB 10 - Veterinärmedizinde_DE
local.opus.fachgebietVeterinärmedizinde_DE
local.opus.id12328
local.opus.instituteInstitute of Veterinary Anatomy, Histology and Embryologyde_DE
local.source.freetextBMC Molecular Biology 16:23de_DE
local.source.urihttps://doi.org/10.1186/s12867-015-0051-7

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