Willkommen bei JLUpub
JLUpub ist das institutionelle Repositorium der Justus-Liebig-Universität.
JLUpub bietet Mitgliedern und Angehörigen der Universität die Möglichkeit neben wissenschaftlichen Dokumenten auch Forschungsdaten elektronisch zu veröffentlichen und dauerhaft zugänglich zu machen. Alle Veröffentlichungen erhalten einen Digital Object Identifier (DOI) und werden über nationale und internationale Bibliothekskataloge sowie Suchmaschinen nachgewiesen und auffindbar.
Neue Veröffentlichungen:
Item type: Item , Characterization of Lineage-Traced Sftpc progenitor cells in the Bronchi (LTS-B) during lung homeostasis and after injury in mice(2025) Noori, AfshinThis dissertation investigates a novel progenitor population in the lung that was identified through Sftpc lineage-tracing experiments. This population resides in the bronchi and is distinct from classical AT2 cells. Although AT2 cells have long been considered the primary source of alveolar regeneration, lineage-tracing studies using SftpcCreERT2 mice combined with Tomatoflox and SftpcGFP reporters revealed an additional population of tdTom⁺ GFP⁻ cells localized to the bronchial epithelium. This population is termed Lineage-Traced Sftpc Cells in the Bronchi (LTS-B). These cells represent approximately 1.6% of epithelial cells and occupy a unique niche on the apical side of bronchial smooth muscle. Interestingly, transcriptomic analysis revealed a hybrid identity with reduced AT2 signatures, enrichment of ciliated and club cell markers, activation of PI3K/Akt signaling, and reduced Fgfr2b signaling. These findings establish LTS-Bs as a distinct progenitor pool. Further analysis using single-cell RNA sequencing revealed heterogeneity within this population and identified two distinct subtypes of LTS-B based on transcription. One cluster expresses FoxJ1, Tpp3, and markers, resembling ciliated cells. The other cluster expresses Sftpc and Abca3, resembling alveolar cells. This dual identity underscores their transitional nature, linking airway- and alveolar-like fates. To study epithelial injury and repair dynamics, precision-cut lung slices (PCLS) were used in conjunction with in vivo naphthalene-induced injury. The PCLS model reproduced hallmarks of acute epithelial stress, including rapid declines in Sftpc, Epcam, and Scgb1a1 expression. This is followed by a partial recovery of mesenchymal and growth factor markers, such as Fgf10 and Acta2. This reflects the initiation of repair programs. These results validate PCLS as a powerful ex vivo system for studying progenitor cell behavior. In this context, LTS-B cells expanded in response to injury, both in vivo and ex vivo. Live imaging revealed their motility and migration from the bronchi toward the alveolar area. Next, transcriptomic profiling revealed that LTS-B cells, upon 8 days of PCLS culture, adopt an alveolar differentiation intermediate (ADI)-like state, positioning them as transitional progenitors. Treating PCLS with FGF10 and CHIR99021 partially induced LTS-B differentiation into SftpcGFP⁺ cells, suggesting their potential to contribute to alveolar lineages. Comparative analyses revealed that LTS-Bs are distinct from other progenitor pools, including bronchioalveolar stem cells (BASCs), lineage-negative epithelial progenitors (LNEPs), and basal or club cell populations. Instead of being a variant of these cells, LTS-Bs constitute an unrecognized progenitor pool with a unique identity, localization, and behavior. Preliminary evidence from human idiopathic pulmonary fibrosis (IPF) samples suggests that LTS-B-like cells may exist in humans as well, sharing transcriptional features with the murine population. These findings raise the possibility that LTS-Bs participate in both physiological regeneration and maladaptive remodeling during chronic lung disease. In summary, this dissertation identifies and characterizes LTS-B as a rare, bronchi-localized progenitor population that expands during injury and adopts an ADI-like transitional state. Under regenerative cues, this population can partially differentiate toward AT2 fate. Through the establishment of PCLS as a robust ex vivo injury model and the integration of transcriptomic approaches with functional assays, this study broadens our comprehension of epithelial plasticity in the lung and challenges the prevailing notion that AT2 cells are the sole facilitators of alveolar repair. The discovery of LTS-B highlights the complexity of progenitor hierarchies in the lung and opens new avenues for regenerative strategies. If equivalent cells exist in humans, they could be promising therapeutic targets for diseases such as IPF, COPD, and ARDS. However, dysregulated activation of these progenitors could contribute to pathological fibrosis. By identifying LTS-B, this dissertation provides a framework for rethinking epithelial regeneration and emphasizes the importance of exploring diverse progenitor pools in lung biology and disease.Item type: Item , Gegen jede Sterbeethik(2026-02-05) Duckheim, SimonItem type: Item , BNEreal - Ein interdisziplinäres Projekt der Biologie- und Mathematikdidaktik(2026-02-05) Schmidt, Elvira; Lengnink, Katja; Brück, JohannaBildung für nachhaltige Entwicklung (BNE) ist eine zentrale interdisziplinäre Aufgabe in der Lehrkräftebildung der JLU Gießen, wird jedoch bislang überwiegend disziplinär vermittelt. Im Reallabor BNEreal arbeiten Lehramtsstudierende, Wissenschaftler:innen und Praxisakteur:innen gemeinsam an Nachhaltigkeitsthemen der JLU und der Stadt Gießen. Sie entwickeln didaktisch-methodische Lernangebote, etwa eine digitale Stadtrallye, und reflektieren ihre Ergebnisse. Die Publikation stellt Konzept, Ergebnisse und zentrale Gelingensbedingungen des Projekts vor.Item type: Item , A comparison of drought responses in wild wheat relatives and domesticated wheat grown under irrigated and rainfed field conditions(2025) Pantha, Sumitra; Kilian, Benjamin; Özkan, Hakan; Zeibig, Frederike; Frei, MichaelContext: Domestication and breeding processes for developing modern wheat plants from diverse wild relatives and landraces have had unintended effects of loss of genetic diversity. This reduction in genetic variation undermines the ability of modern wheat cultivars to tolerate environmental stresses such as drought. Wheat wild relatives possess untapped genetic potential for tolerating abiotic stress, especially drought. Yet, their morpho-physiological adaptation and drought stress resilience mechanisms remain underexplored. Objective: This study aimed to investigate the adaptive responses of plants within the Triticum spp. genepool, encompassing wheat wild relatives, landraces, and modern cultivars to drought stress under rainfed and irrigated field conditions. Methods: From an initial pool of 110 genotypes screened during the first growing season in 2022, 20 best performing genotypes, including modern cultivars for comparison, were selected for a second growing season in 2023 based on their relative yield performance. Two different treatment conditions, irrigated and rainfed, were applied during both growing seasons. This experiment observed single plants per replicate. Multiple parameters, including days to heading and flowering, plant height, number of spikes per plant, spike length, spike weight per plant, straw weight per plant, aboveground biomass per plant, grain yield per plant, thousand kernel weight, harvest index, stomatal conductance, and vegetation indices, were assessed on the selected genotypes. Results: Taking averages measured across both growing seasons, we observed significant genotypic variation across several parameters: days to heading and flowering, plant height, number of spikes per plant, spike length, spike and straw weight per plant, aboveground biomass per plant, grain yield per plant, thousand kernel weight, harvest index, stomatal conductance, and vegetation indices. Water stress during the rainfed treatment significantly reduced grain yield (by 21 %) and stomatal conductance (by 45 %). Stomatal conductance was associated with grain yield and yield-related traits under rainfed conditions. Diverse physiological drought tolerance mechanisms associated with stomatal regulation were identified, revealing genotype-specific responses to drought stress. Genotypes such as T. dicoccoides (G242), T. urartu (G45), T. boeoticum (G27) and T. araraticum (G221) exhibited isohydric adaptation, whereas T. monococcum sinskajae (G89) and T. durum cv. Sambadur (G41) exhibited anisohydric adaptation. Conclusion: Some genotypes of T. dicoccoides, T. urartu, T. boeoticum and T. araraticum exhibited isohydric adaptation, while T. monococcum sinskajae and T. durum cv. Sambadur exhibited anisohydric adaptation under drought stress which needs further verification. These genotypes can serve as donors for introducing drought tolerance traits within wheat improvement programs.Item type: Item , Antidiabetic and antioxidant profiling of 67 African trifoliate yam accessions by planar on-surface assays versus in vitro assays(2025) Aiyedun, Priscilla O.; Sonibare, Mubo A.; Gueye, Badara; Albach, Dirk C.; Heil, Julia; Morlock, GertrudBTrifoliate yam (Dioscorea dumetorum) is traditionally used to treat diabetics in Nigeria. However, almost no information is available on its antidiabetic constituents and their natural variance. Hence, the activity of methanolic tuber extracts of 67 trifoliate yam accessions from the largest collection in Africa was proven by four colorimetric antidiabetic and antioxidant in vitro assays, as diabetes is also linked with oxidative stress. For the first time, selected accessions were also analyzed by planar bioactivity profiling. It has a comparatively higher, more differentiated information content, is more sustainable in terms of material consumption, and enables straightforward compound prioritization and characterization. Up to a dozen individual antioxidant zones were revealed as well as one prominent zone inhibiting α-glucosidase and α-amylase. The latter inhibition zone was tentatively assigned to palmitic, linoleic, oleic, linolenic, oxo-nonanoic fatty acids by direct elution to heated electrospray ionization high-resolution mass spectrometry.