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JLUpub ist das institutionelle Repositorium der Justus-Liebig-Universität.
JLUpub bietet Mitgliedern und Angehörigen der Universität die Möglichkeit neben wissenschaftlichen Dokumenten auch Forschungsdaten elektronisch zu veröffentlichen und dauerhaft zugänglich zu machen. Alle Veröffentlichungen erhalten einen Digital Object Identifier (DOI) und werden über nationale und internationale Bibliothekskataloge sowie Suchmaschinen nachgewiesen und auffindbar.
Neue Veröffentlichungen:
Molecular composition of skeletal muscle in infants and adults: a comparative proteomic and transcriptomic study
(2024) Schaiter, Alexander; Hentschel, Andreas; Kleefeld, Felix; Schuld, Julia; Umathum, Vincent; Procida-Kowalski, Tara; Nelke, Christopher; Roth, Angela; Hahn, Andreas; Krämer, Heidrun H.; Ruck, Tobias; Horvath, Rita; van der Ven, Peter F. M.; Bartkuhn, Marek; Roos, Andreas; Schänzer, Anne
Making plaque assessment easier – a validation study of simplified versions of the Marginal Plaque Index
(2024) Weik, Ulrike; Eidenhardt, Zdenka; Deinzer, Renate
Background: The assessment of plaque indices may be time-consuming and error-prone. Simplification of these indices may increase their utility without compromising their validity. The aim of this study was to evaluate the validity of two simplified versions of the Marginal Plaque Index (MPI).
Methods: Two simplified versions of the MPI as well as the Plaque Control Record (PCR) were derived from full-scale MPI assessments in two studies with four age groups (N = 42 10-year-olds; N = 24 15 year-olds; N = 53 university students (18y-33y); N = 66 parents (32y-57y). Correlations with the Turesky modification of the Quigley-Hein Index (TQHI) and the Papillary Bleeding Index (PBI) were calculated.
Results: The indices derived from the MPI showed high convergence with each other (all r ≥ 0.94) and with the TQHI (r ≥ 0.80). The concurrent validity of the MPI with the PBI was equal to that of the TQHI in all age groups. The simplified versions of the MPI and the PCR show a lower convergent validity with the PBI than the MPI within parents (p < 0.05). In the other age groups, their convergent validity was equal to that of the MPI.
Discussion: Simplification of the MPI does not affect its convergent validity with other plaque indices but may reduce its concurrent validity with the PBI in middle-aged adults.
Marginal Quality and Wear of Bulk-Fill Composites: Differences Between Dentitions
(2024) Hofmann, Maria; Wolf, Emma; Lücker, Susanne; Frankenberger, Roland; Wöstmann, Bernd; Krämer, Norbert
Purpose: The aim of this study was to evaluate the marginal quality and wear of bulk-fill composite resins (BFs) for Class-II restorations of primary and permanent molars in comparison to a conventionally layered composite resin (RC) and to compare the results of the two dentitions.
Materials and methods: Eighty (40 primary and 40 permanent) extracted molars received standardized Class-II cavity preparations and were restored with either one of two flowable BFs, one of two high viscous BFs, or a composite resin (RC). Thermomechanical loading (TML; 2,500 cycles +5°C/+55°C; 100,000 cycles, 50N, 1.67Hz) followed. A quantitative marginal analysis using SEM images and a profilometric quantification of two-body wear were carried out using replicas. ANOVA, Kruskal-Wallis, Mann-Whitney U, and Wilcoxon signed-rank tests were used for statistical analysis (P < 0.05).
Results: For both dentitions, a significant reduction of perfect margins was observed after TML (P < 0.02). For the primary dentition, the flowable BFs showed significantly less perfect margins than all high viscous materials (P < 0.005). For the permanent dentition, RC showed significantly fewer gaps than the flowable BFs (P < 0.04). Regarding wear, within the dentitions, no significant differences could be computed between groups with regard to the maximum height loss (P < 0.05).
Conclusion: All of the investigated bulk-fill composite resins showed satisfactory in-vitro results for both tested parameters in primary and permanent teeth, with a superiority of the high-viscosity materials in terms of marginal quality.
A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation
(2024) Muhammad, Abubakar; Sarkadi, Zsuzsa; Mazumder, Agnisrota; Ait Saada, Anissia; van Emden, Thomas; Capella, Matias; Fekete, Gergely; Suma Sreechakram, Vishnu N; Al-Sady, Bassem; Lambert, Sarah A E; Papp, Balázs; Barrales, Ramón Ramos; Braun, Sigurd
Heterochromatin plays a critical role in regulating gene expression and maintaining genome integrity. While structural and enzymatic components have been linked to heterochromatin establishment, a comprehensive view of the underlying pathways at diverse heterochromatin domains remains elusive. Here, we developed a systematic approach to identify factors involved in heterochromatin silencing at pericentromeres, subtelomeres and the silent mating type locus in Schizosaccharomyces pombe. Using quantitative measures, iterative genetic screening and domain-specific heterochromatin reporters, we identified 369 mutants with different degrees of reduced or enhanced silencing. As expected, mutations in the core heterochromatin machinery globally decreased silencing. However, most other mutants exhibited distinct qualitative and quantitative profiles that indicate heterochromatin domain-specific functions, as seen for example for metabolic pathways affecting primarily subtelomere silencing. Moreover, similar phenotypic profiles revealed shared functions for subunits within complexes. We further discovered that the uncharacterized protein Dhm2 plays a crucial role in heterochromatin maintenance, affecting the inheritance of H3K9 methylation and the clonal propagation of the repressed state. Additionally, Dhm2 loss resulted in delayed S-phase progression and replication stress. Collectively, our systematic approach unveiled a landscape of domain-specific heterochromatin regulators controlling distinct states and identified Dhm2 as a previously unknown factor linked to heterochromatin inheritance and replication fidelity.
Association of PH-Targeted Therapy and Survival in Precapillary PH with mPAP between 21 and 24 mmHg
(2024) Yogeswaran, Athiththan; Fünderich, Meike; Olschewski, Horst; Kovacs, Gabor; Kiely, David G; Lawrie, Allan; Hassoun, Paul M.; Balasubramanian, Aparna; Konswa, Ziad; Pepke-Zaba, Joanna; Cannon, John; Wilkins, Martin R.; Howard, Luke; Ghofrani, Hossein Ardeschir; Grimminger, Friedrich; Seeger, Werner; Tello, Khodr
Introduction: The definition of pulmonary hypertension (PH) was recently changed and led to a new subset of PH patients with mildly impaired pulmonary haemodynamics, characterised by a mean pulmonary artery pressure (mPAP) of 21–24 mmHg and with a pulmonary vascular resistance (PVR) >2 WU. We evaluated the association of PH-targeted therapy and outcome in mild precapillary PH using the PVRI GoDeep meta-registry.
Methods: All patients with mild precapillary PH (mPAP 21–24 mmHg, pulmonary arterial wedge pressure ≤15 mmHg and PVR >2 WU) diagnosed with pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH) were enrolled. Patients were considered as “treated” if PH-targeted therapy was initiated within 6 months of diagnostic right heart catheterisation. Various statistical models, including in-depth sensitivity analyses, were used to examine the association between PH-targeted therapy and transplant-free survival.
Results: 132 patients with group 1 or group 4 mild PH were identified, of whom 34 patients received PH-targeted therapy. There were no differences in baseline haemodynamics between untreated and treated groups, whereas treated patients suffered more frequently from renal comorbidities and required long-term oxygen treatment more often. Most prescribed were phosphodiesterase-5-inhibitors. PH-targeted therapy was associated with significantly higher survival rates. Cox-regression analyses revealed significantly reduced hazard ratios among treated patients adjusted for various confounders. Subgroup analyses in PAH (n=78) similarly indicated higher survival rates and reduced hazard ratios in treated patients.
Conclusion: PH-targeted therapy may be associated with improved survival in PAH and CTEPH patients with mild PH. To mitigate potential bias of the results due to the retrospective study design, randomised controlled trials are warranted.