The data are supplementary materials for the research article "Genome-wide associations for heat stress response infer potential candidate genes underlying milk fatty acid composition in dairy cattle"
The objective of this study was to define FA heat stress indicators, considering FA breeding values in response to temperature-humidity index ... (THI) alterations. Genome-wide association studies (GWAS) were conducted to annotate potential candidate genes for FA traits under different climate conditions. The phenotypic dataset consisted of 39,600 test-day milk FA records from 5,757 first-lactation Holstein dairy cows. The FA traits were C18:0, poly-unsaturated fatty acids (PUFA), saturated fatty acids (SFA) and unsaturated fatty acids (UFA). Meteorological data from the weather station in closest herd distance were used for the calculation of maximum hourly daily THI.
The Supplementary Materials include two Figures:
Figure S1. Manhattan plot of -log10 P-values of the tested SNP markers for C18:0 (A), poly-unsaturated fatty acids (B), saturated fatty acids (C) and unsaturated fatty acids (D) in different lactation stages under thermoneutral conditions (TNC), under heat stress conditions (HSC) and for heat stress response (HSR). The red dashed line is the significance threshold for the Bonferroni correction. Genomic estimated breeding values were used as pseudo-phenotypes.
Figure S2. Supplemental Figure S2. Venn diagrams for the number of significant SNP markers and genes associated with heat stress response overlapping across FA traits (A), associated with heat stress response for FA traits overlapping across lactation stages (B) and associated with FA traits overlapping across environmental conditions (C). Genomic estimated breeding values were used as pseudo-phenotypes. FA traits are C18:0, poly-unsaturated fatty acids (PUFA), saturated fatty acids (SFA) and unsaturated fatty acids (UFA). Lactation stages are the first (S1), the second (S2) and the third (S3) stages. Environmental conditions are thermoneutral conditions (TNC), heat stress conditions (HSC) and heat stress response (HSR).
