Microscopy Imaging Data for “Chronic lung disease induced brain microenvironment changes: implications in pre-metastatic niche formation”

Abstract

Microscopy imaging data related to the “Chronic lung disease induced brain microenvironment changes: implications in pre-metastatic niche formation” manuscript by Salik et al., 2024

Folders (uploaded as zip files): HIF1a and HIF2a IHC (25,08 GB) Podocalyxin and Iba1 staining-FFPE sections (32,79 GB) Podocalyxin-Fibrinogen co-staining (54,15 GB) Representative images (403,81 MB) Videos (28,92 MB)

The folders contain image files and corresponding metadata files. Imaging was performed on a CQ1 spinning disc confocal microscope (Yokogawa Life Science, Tokyo, Japan; Cenibra GmbH, Bramsche, Germany) using 405/488/561 nm lasers and 20x, or 40x objective, or on an Axio Scan.Z1 slide scanner (Carl Zeiss Microscopy GmbH, Jena, Germany) using a 20x objective and the ZEN 2.3 software for acquisition. Podocalyxin staining folders contain 4 images that are DAPI, A555 (podocalyxin staining), merge and binary version of podocalyxin staining. Iba1 staining folders contain 5 images that are DAPI, A555 (Iba1 staining), merge, Iba1+ cell count, and DAPI count. Note on Podocalyxin and Iba1 staining - 250um sections: Z-projections are available in the folders as PNG files, original data (>10 Gb for each sample) are available on request.

Abbreviations: NO (normoxia): Control Hox (hypoxia): CMH (chronic mild hypoxia) RA (room air): Control SE (smoke exposure) or CS (cigarette smoke): S-PH or S-Em (tobacco-smoke exposure induced pulmonary hypertension or also emphysema, after 3-month or 8-month exposure, respectively)