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Item type: Item , Cellular immune responses of bovine polymorphonuclear neutrophils to Calicophoron daubneyi(2025) Silva, Liliana M. R.; López-Osorio, Sara; Peixoto, Raquel; Zhou, Ershun; Espinosa, Gabriel; Gärtner, Ulrich; Taubert, Anja; Conejeros, Iván; Hermosilla, CarlosItem type: Item , Retrospective evaluation of autotransfusion using a cell saver device versus allotransfusion in the perioperative management of acute hemoperitoneum in 43 dogs (2017–2021)(2025) Blunschi, Fabienne; Gluding, Dennis; Hassdenteufel, Esther; Schneider, Matthias; Lehmann, HendrikBackground: Cell saver (CS) technology is an increasingly popular approach for autotransfusion in small animal veterinary medicine for the treatment of patients with abdominal hemorrhagic effusion. Objective: To evaluate the utility, effectiveness, and safety of autotransfusions collected with a CS device and to assess whether the use of the CS device reduces the demand for allogenic blood transfusions. Materials and methods: Retrospective study of dogs with acute hemoperitoneum of splenic origin treated surgically. Dogs were grouped by the type of transfusion received: allo- and autotransfusion (AA), allotransfusion only (AO), autotransfusion only (CS), and no transfusion (NT). Differences in changes of laboratory parameters (hematocrit and lactate), transfusion volume, and outcomes were analyzed across groups. Results: Forty-three dogs were included. Twenty-seven (62.8%) suffered from hemangiosarcoma, and 16 (37.2%) had a benign cause of hemoperitoneum. The classification into blood transfusion groups was as follows: 7/43 (16.3%) in the AA-group, 11/43 (25.6%) in the AO-group, 11/43 (25.6%) in the CS-group and 14/43 (32.6%) in the NT-group. Increase in hematocrit over time was similar in all subgroups that received any form of blood transfusion (AA-, AO-, CS-group). Total volume of transfused blood (autologous and allogenic) was significantly higher in the AA-group (median 54.0mL/kg, range 24.7–126.5mL/kg) than in the AO-group (median 7.6mL/kg, range 4.6–13.5mL/kg, p = 0.01) but not the CS-group (median 23.8mL/kg, range 14.1–50.0mL/kg, p = 0.22). No difference was found for the volume of allogenic blood transfused between the AA-group (median 9.4mL/kg, range 5.0–16.2mL/kg) and AO-group (median 7.6mL/kg, range 4.6–13.5mL/kg) (p = 0.68). The use of the CS device did not adversely affect the time from presentation to surgery, the duration of surgery, or the outcomes. Discussion: The use of autologous blood transfusions obtained by CS device in dogs suffering from acute hemoperitoneum caused by a benign or malignant splenic disorder appeared safe and effective in the cases described. And therefore may emphasize its further application as an addition or alternative to traditional allogenic blood transfusions.Item type: Item , Perspectives on the adoption of black-soldier fly larvae for animal feed among livestock farmers in Sub-Saharan Africa(2025) Nyamuhirwa, Dieu-Merci Akonkwa; Feleke, Shiferaw; Dontsop Nguezet, Paul-Martin; Sissoko, Dioukou; Moussa, Bokar; Kouakou, Abel-Gautier; Zakari, Seydou; Abdoulaye, Tahirou; Manyong, VictorIntroduction: Black soldier fly larvae (BSFL) are increasingly promoted as cost-effective, and environmentally friendly source of protein in animal nutrition. However, there is limited information about farmers' adoption rates and the factors influencing their adoption decisions in Sub-Saharan Africa (SSA). This article assesses livestock farmers' actual and potential adoption rates of BSFL and determinants of their adoption decisions. Methods: In this paper, we used the treatment effect framework approach on data collected from 1,885 fish, poultry, and pig smallholder farmers in five cities of four West and Central African countries, including Kinshasa and Bukavu in the Democratic Republic of Congo (DRC), Accra in Ghana, Bamako in Mali, and Niamey in Niger. Results and discussion: The results show that about 20 percent of surveyed farmers are aware of BSFL as a protein source in animal feed, and the actual adoption rate of BSFL is four percent. However, the treatment effect analysis showed that the adoption rate could quadruple if all farmers were aware. This result suggests that successful awareness creation can boost the actual adoption of BSFL, which currently stands at four percent. The awareness creation should target educated livestock farmers with access to group membership, credit, extension services, and diversified income sources to influence their decisions to adopt BSFL as a source of protein in animal feed.Item type: Item , Intracrine Formation of Steroid Hormones in Breast Cancer, Epidermal Keratinocyte, Dermal Fibroblast, and Adipocyte Cell Lines Measured by LC-MS/MS(2025) Karakus, Emre; Schmid, Andreas; Schäffler, Andreas; Wudy, Stefan A.; Geyer, JoachimPeripheral tissues such as skin and adipose tissue play a crucial role in the intracrine formation of sex steroid hormones, complementing the endocrine and paracrine systems. These mechanisms involve the conversion of dehydroepiandrosterone (DHEA) and its sulfated form—DHEAS—into potent androgenic and estrogenic hormones. In vitro studies using tissue-specific cell lines are essential for unraveling the complex intracrine synthesis of these hormones. This study examined the formation of DHEA, androstenedione (A4), testosterone (T), dihydrotestosterone (DHT), and estradiol (E2) from DHEAS in four cell lines: MCF-7 breast cancer cells, HaCaT keratinocytes, human dermal fibroblasts (HDF), and 3T3-L1 preadipocytes and mature adipocytes, using liquid chromatography–mass spectrometry (LC-MS/MS). MCF-7 cells converted DHEAS to DHEA, A4, T, E2, and DHT, while HaCaT cells produced all these steroids except DHT. Mature 3T3-L1 adipocytes produced DHEA, A4, T, and DHT. By contrast, HDF and 3T3-L1 preadipocytes converted DHEAS only to DHEA and A4. This study highlights the vital role of peripheral tissues, such as skin and adipose tissue, for the intracrine formation of sex hormones and underlines the crucial role of in vitro cell culture models to analyze such effects. The data shed light on the significant impact of androgen metabolism in skin and adipose tissue, which is of great relevance for aging, wound healing, obesity, and lipid metabolism.Item type: Item , Screening of Piglets for Signs of Inflammation and Necrosis as Early Life Indicators of Animal Health and Welfare Hazards(2025) Koenders-van Gog, Karien; Wijnands, Thomas; Lechner, Mirjam; Reiner, Gerald; Fink-Gremmels, JohannaEnsuring animal health and well-being requires animal-based measures for early and direct intervention at the point of care. Insight into the pathophysiology of Swine Inflammation and Necrosis Syndrome (SINS) has led to a non-invasive, clinical scoring system that can be used in daily practice. This provides information on the cause of observed lesions for direct intervention in affected herds. The aim of the current study was to re-evaluate the practicality of the SINS scoring system under field conditions. In addition, this is the first study to provide insight into the prevalence of SINS on Dutch farms. This study involved the scoring of 5958 piglets from 20 visits on 13 farms randomly selected by a veterinary practice in the Netherlands. The results showed that up to 64.1% of the piglets had visible inflammatory alterations in different body parts within the first seven days of life. Sow rectal temperature, signs of coprostasis and water intake during pregnancy were significantly (p ≤ 0.05) correlated with the prevalence of SINS in piglets. In conclusion, the SINS scoring system is an easy-to-use, non-invasive diagnostic tool that summarises animal-based observations at the point of care, providing a valuable communication tool between farmers, nutritionists and veterinarians in their efforts to improve animal health and welfare.