Publikationen im Open Access gefördert durch die UB

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Auflistung Publikationen im Open Access gefördert durch die UB nach Auflistung nach Fachbereich/Einrichtung "FB 10 - Veterinärmedizin"

Gerade angezeigt 1 - 20 von 366
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    A contribution to age determination of cheetahs (Acinonyx jubatus) based on radiographic analysis of the skull and postcranial morphology.
    (2019) Schmidt, Martin J; Steenkamp, Gerhard; Failing, Klaus; Caldwell, Peter; Kirberger, Robert M
    The aim of this retrospective cross-sectional study was to present comprehensive information about the age-dependent change of skeletal characteristics in captive cheetahs with known age and to assess the benefit of these variables for age estimation in this species. Radiographs of 162 known-age captive and semi-captive cheetahs were retrospectively examined and age-related changes of skull, axial and appendicular skeletal systems were documented. Metric and non-metric variables were used. These parameters were checked for the best correlation with age using a multiple stepwise regression analysis. An overview about the time frames, in which ossification centers appeared and physeal closure occurred is presented. Multiple stepwise regression analysis revealed the status of closure of the coronal suture, the maximum length of the frontal sinus, the condylobasal-, hard palate, and facial length are most significantly correlated with age. Together with the pulp size of the upper canine, these values can be used for an age approximation in cheetahs.
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    A genome-wide scan study identifies a single nucleotide substitution in MC1R gene associated with white coat colour in fallow deer (Dama dama)
    (2020) Reiner, Gerald; Weber, Tim; Nietfeld, Florian Georg; Fischer, Dominik; Wurmser, Christine; Fries, Ruedi; Willems, Hermann
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    A genome-wide scan study identifies a single nucleotide substitution in the tyrosinase gene associated with white coat colour in a red deer (Cervus elaphus) population
    (2020) Reiner, Gerald; Tramberend, Kirsten; Nietfeld, Florian; Volmer, Klaus; Wurmser, Christine; Fries, Ruedi; Willems, Hermann
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    A placebo-controlled, double-blind study evaluating the effect of orally administered polyunsaturated fatty acids on the oclacitinib dose for atopic dogs
    (2024) Schäfer, Laura; Thom, Nina
    Background: Supplementation of polyunsaturated fatty acids (PUFA) enables dose reduction of prednisolone and ciclosporin in canine atopic dermatitis (cAD). Objective: To determine if oral administration of PUFA reduces the dose of oclacitinib in cAD. Animals: Twenty-two client-owned dogs with cAD receiving oclacitinib. Materials and Methods: Dogs received a fish oil product (PUFA) or paraffin oil (placebo) for 16 weeks. Owners adjusted the oclacitinib dose according to daily pruritus assessments. On Day (D)0, D56 and D112, Canine Atopic Dermatitis Extent and Severity Index, fourth iteration (CADESI-04), pruritus Visual Analog Scale (PVAS), quality-of-life score (QoL), Global Assessment (GA), quality-of-coat (QoC) and adverse events were recorded. Results: Mean daily oclacitinib dose was significantly reduced in the PUFA group from 0.51 ± 0.20 mg/kg/24 h (D0) to 0.19 ± 0.14 mg/kg/24 h (D85–112; p < 0.00001) and not in the placebo group (D0: 0.70 ± 0.33 mg/kg/24 h; D85–112: 0.53 ± 0.35 mg/kg/24 h, p = 0.5422). CADESI-04 did not change over time or differ between groups. PVAS was significantly lower in the PUFA group (2.8 ± 1.5) compared to placebo (4.2 ± 1.6) at D112 (p = 0.0375). QoL and QoC improved only in the PUFA group (QoL: D0: 20 ± 7, D112: 12 ± 5, p = 0.0057; QoC: D0: 0 ± 0.5, D112: 1 ± 0.5, p = 0.0410). GA on D112 was higher in the PUFA group (p = 0.008). No adverse events were observed. Conclusion: Oral supplementation of PUFA allowed dose reduction of oclacitinib and improved PVAS, QoL, QoC and GA. The use of PUFA is recommended and was safe in the atopic study dogs receiving oclacitinib.
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    A rare case of multicentric eosinophilic granulocytic sarcoma as cause of hindlimb weakness and non-regenerative anaemia in a minipig
    (2021) Weyrich, Angelika; Becker, Sabrina; Vienenkötter, Julia; Reiner, Gerald; Herden, Christiane
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    A special issue on “New technologies in parasitology”
    (2022) Brehm, Klaus; Taubert, Anja; Grevelding, Christoph G.
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    Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome
    (2022) Theuerkauf, Kim; Obach-Schröck, Carmen; Staszyk, Carsten; Moritz, Andreas; Roscher, Katja A.
    In humans, activated platelets contribute to sepsis complications and to multiple organ failure. In our prospective analytical study of cases of the equine systemic inflammatory response syndrome (SIRS), we adapted a standard human protocol for the measurement of activated platelets and platelet-leukocyte aggregates (PLAs) in equine platelet-leukocyte-rich plasma (PLRP) by flow cytometry, and we investigated the hypothesis that activated platelets and PLAs are increased in clinical cases of SIRS. We included 17 adult horses and ponies fulfilling at least 2 SIRS criteria, and 10 healthy equids as controls. Activation of platelets was determined by increased expression of CD62P on platelets. Activated platelets and PLAs were measured before and after in vitro activation of platelets with collagen. Median expression of CD62P on platelets was significantly increased after activation in the control group: 1.45% (interquartile range [IQR]: 1.08–1.99%) initially versus 8.78% (IQR: 6.79–14.78%, p = 0.002) after activation. The equids with SIRS had significantly more activated platelets and PLAs in native PLRP than controls: CD62P 4.92% (median, IQR: 2.21–12.41%) versus 1.45% in controls (median, IQR: 1.08–1.99%, p = 0.0007), and PLAs 4.16% (median, IQR: 2.50–8.58%) versus 2.95% in controls (median, IQR: 1.57–3.22%, p = 0.048). To our knowledge, increased platelet activation and PLAs have not been demonstrated previously with flow cytometry in clinical cases of equine SIRS.
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    Activation of the inflammatory transcription factor nuclear factor interleukin-6 during inflammatory and psychological stress in the brain
    (2013) Fuchs, Franziska; Damm, Jelena; Gerstberger, Rudiger; Roth, Joachim; Rummel, Christoph
    BACKGROUND:The transcription factor nuclear factor interleukin 6 (NF-IL6) is known to be activated by various inflammatory stimuli in the brain. Interestingly, we recently detected NF-IL6-activation within the hypothalamus-pituitary-adrenal (HPA)-axis of rats after systemic lipopolysaccharide (LPS)-injection. Thus, the aim of the present study was to investigate whether NF-IL6 is activated during either, inflammatory, or psychological stress in the rat brain. METHODS:Rats were challenged with either the inflammatory stimulus LPS (100mug/kg, i.p.) or exposed to a novel environment. Core body temperature (Tb) and motor activity were monitored using telemetry, animals were killed at different time points, brains and blood removed, and primary cell cultures of the anterior pituitary lobe (AL) were investigated. Analyses were performed using immunohistochemistry, RT-PCR, and cytokine-specific bioassays. RESULTS:Stress stimulation by a novel environment increased NF-IL6-immunoreactivity (IR) in the pituitary´s perivascular macrophages and hypothalamic paraventricular cells and a rise in Tb lasting approximately 2h. LPS stimulation lead to NF-IL6-IR in several additional cell types including ACTH-IR-positive corticotrope cells in vivo and in vitro. Two other proinflammatory transcription factors, namely signal transducer and activator of transcription (STAT)3 and NFkappaB, were significantly activated and partially colocalized with NF-IL6-IR in cells of the AL only after LPS-stimulation, but not following psychological stress. In vitro NF-IL6-activation was associated with induction and secretion of TNFalpha in folliculostellate cells, which could be antagonized by the JAK-STAT-inhibitor AG490. CONCLUSIONS:We revealed, for the first time, that NF-IL6 activation occurs not only during inflammatory LPS stimulation, but also during psychological stress, that is, a novel environment. Both stressors were associated with time-dependent activation of NF-IL6 in different cell types of the brain and the pituitary. Moreover, while NF-IL6-IR was partially linked to STAT3 and NFkappaB activation, TNFalpha production, and ACTH-IR after LPS stimulation; this was not the case after exposure to a novel environment, suggesting distinct underlying signaling pathways. Overall, NF-IL6 can be used as a broad activation marker in the brain and might be of interest for therapeutic approaches not only during inflammatory but also psychological stress.
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    Adaptation of Campylobacter field isolates to propionic acid and sorbic acid is associated with fitness costs
    (2021) Peh, E.; Kittler, S.; Seinige, D.; Valero, A.; Kehrenberg, C.
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    Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine
    (2015) Arnhold, Stefan; Wenisch, Sabine
    Adipose tissue derived stem cells (ASCs) are mesenchymal stem cells which can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, which is easy accessible and the cells can be obtained in large numbers, cultivated and expanded in vitro and prepared for tissue engineering approaches, especially for skeletal tissue repair. In the recent years this cell population has attracted a great amount of attention among researchers in human as well as in veterinary medicine. In the meantime ASCs have been well characterized and their use in regenerative medicine is very well established. This review focuses on the characterization of ASCs for their use for tissue engineering approaches especially in veterinary medicine and also highlights a selection of clinical trials on the basis of ASCs as the relevant cell source.
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    Aetiology and 30-Year Long-Term Outcome of Children with Cardiomyopathy Necessitating Heart Transplantation
    (2020) Zschirnt, Martin; Thul, Josef; Akintürk, Hakan; Valeske, Klaus; Schranz, Dietmar; Skrzypek, Susanne; Müller, Matthias; Jux, Christian; Hahn, Andreas; Rupp, Stefan
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    Age Dependent Hypothalamic and Pituitary Responses to Novel Environment Stress or Lipopolysaccharide in Rats
    (2018) Koenig, Sandy; Bredehöft, Janne; Perniss, Alexander; Fuchs, Franziska; Roth, Joachim; Rummel, Christoph
    Previously, we have shown that the transcription factor nuclear factor interleukin (NF-IL)6 can be used as an activation marker for inflammatory lipopolysaccharide (LPS)-induced and psychological novel environment stress (NES) in the rat brain. Here, we aimed to investigate age dependent changes of hypothalamic and pituitary responses to NES (cage switch) or LPS (100µg/kg) in two and 24 months old rats. Animals were sacrificed at specific time points, blood and brains withdrawn and analyzed using immunohistochemistry, RT-PCR and bioassays. In the old rats, telemetric recording revealed that NES-induced hyperthermia was enhanced and prolonged compared to the young group. Plasma IL-6 levels remained unchanged and hypothalamic IL-6 mRNA expression was increased in the old rats. Interestingly, this response was accompanied by a significant upregulation of corticotropin-releasing hormone mRNA expression only in young rats after NES and overall higher plasma corticosterone levels in all aged animals. Immunohistochemical analysis revealed a significant upregulation of NF-IL6-positive cells in the pituitary after NES or LPS-injection. In another important brain structure implicated in immune-to-brain communication, namely, in the median eminence (ME), NF-IL6-immunoreactivity was increased in aged animals, while the young group showed just minor activation after LPS-stimulation. Interestingly, we found a higher amount of NF-IL6-CD68-positive cells in the posterior pituitary of old rats compared to the young counterparts. Moreover, aging affected the regulation of cytokine interaction in the anterior pituitary lobe. LPS-treatment significantly enhanced the secretion of the cytokines IL-6 and TNFalpha into supernatants of primary cell cultures of the anterior pituitary. Furthermore, in the young rats, incubation with IL-6 and IL-10 antibodies before LPS-stimulation led to a robust decrease of IL-6 production and an increase of TNFalpha production by the pituitary cells. In the old rats, this specific cytokine interaction could not be detected. Overall, the present results revealed strong differences in the activation patterns and pathways between old and young rats after both stressors. The prolonged hyperthermic and inflammatory response seen in aged animals seems to be linked to dysregulated pituitary cytokine interactions and brain cell activation (NF-IL6) in the hypothalamus-pituitary-adrenal axis.
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    Age-Dependent Changes of Adipokine and Cytokine Secretion From Rat Adipose Tissue by Endogenous and Exogenous Toll-Like Receptor Agonists
    (2020) Peek, Verena; Neumann, Elena; Inoue, Tomohiro; Koenig, Sandy; Pflieger, Fabian Johannes; Gerstberger, Rüdiger; Roth, Joachim; Matsumura, Kiyoshi; Rummel, Christoph
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    Animal-Assisted Interventions and Animal Welfare - An Exploratory Survey in Germany
    (2023) Ameli, Katharina; Braun, Theresa F.; Krämer, Stephanie
    Background: Animals play a crucial role in social occupational fields. The positive effects of animals are described in theory and practice. However, the significance of animal welfare in animal-assisted intervention settings has not yet been extensively researched, so that the aim of this explorative study was to investigate the perception and significance as well as the understanding of animal welfare and its implementation on the part of professionals working with animals. Methods: In the present project, 270 animal-assisted professionals from Germany were interviewed about their individual perceptions of animal welfare and their implementation of animal welfare with the help of questionnaires with closed questions (5-point agreement scale) and open questions. The quantitative data were analyzed using the statistical software SPSS and MS Excel. The qualitative data were analyzed using thematic coding. Results: The quantitative and qualitative results show that animal welfare poses high importance for people working in animal-assisted interventions. The structure and design of assignments, animal-related aspects and conditions, and education and knowledge are mentioned as generally relevant conditions for ensuring animal welfare from the perspective of animal-assisted intervention practitioners. In addition, different concrete courses of action to ensure animal welfare are described, which are characterized as stopping or changing the setting at different levels. Conclusions: Animal welfare plays a central role for professionals working with animals. However, further studies are necessary in order to record other animal welfare-relevant aspects in the animal-assisted interventions, depending on the respective animal species, and to examine the implementation of animal welfare-related measures.