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dc.contributor.authorWeiterer, Sebastian
dc.contributor.authorSchulte, Dagmar
dc.contributor.authorMüller, Sabrina
dc.contributor.authorKohlen, Thomas
dc.contributor.authorUhle, Florian
dc.contributor.authorWeigand, Markus A.
dc.contributor.authorHenrich, Michael
dc.date.accessioned2022-11-18T09:50:31Z
dc.date.available2014-10-27T14:31:16Z
dc.date.available2022-11-18T09:50:31Z
dc.date.issued2014
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-111536
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9071
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8459
dc.description.abstractThe tracheal epithelium prevents via its highly effective clearance mechanism the contamination of the lower airways by pathogens. This mechanism is driven by ciliary bearing cells which are not only in contact with the gas phase; in addition they are also influenced by inflammatory mediators. These mediators can alter the protective function of the epithelium. Since the pro-inflammatoric cytokine tumor necrosis factor-alpha (TNF-alpha) plays a pivotal role within the inflammatory cascade, we investigated its effect onto the tracheal epithelium measured by its ciliary beat frequency and the particle transport velocity. In organ explant experiments the ciliary beat frequency and the particle transport velocity were measured under the application of TNF-alpha using tracheae from male C57BL6J mice. We observed a dose dependent TNF-alpha induced increase of both particle transport velocity and ciliary beat frequency. Knock out mice experiments made evident that the increase was depended on the expression of tumor necrosis factor receptor 1 (TNF-R1). The increases in ciliary beat frequency as well as the accelerated particle transport velocity were either inhibited by the unspecific serotonin antagonist methysergide or by cyproheptadine a specific 5-HT2 receptor antagonist. Thus, acetylcholine antagonists or nitric oxide synthase (NOS) inhibitors failed to inhibit the TNF-alpha induced activation. In conclusion, TNF-alpha may play a pivotal role in the protection of lower airways by inducing ciliary activity and increase in particle transport velocity via TNF-R1 and 5-HT2 receptor.en
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subject.ddcddc:610de_DE
dc.titleTumor necrosis factor alpha induces a serotonin dependent early increase in ciliary beat frequency and epithelial transport velocity in murine tracheaeen
dc.typearticlede_DE
local.affiliationFB 11 - Medizinde_DE
local.opus.id11153
local.opus.instituteKlinik für Anästhesiologie, Operative Intensivmedizin und Schmerztherapiede_DE
local.opus.fachgebietMedizinde_DE
local.source.urihttps://doi.org/10.1371/journal.pone.0091705
local.source.freetextPLoS ONE 9(3):e91705de_DE


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