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dc.contributor.authorPfeifer, Kathrin
dc.contributor.authorSchaub, Christoph
dc.contributor.authorDomsch, Katrin
dc.contributor.authorDorresteijn, Adriaan
dc.contributor.authorWolfstetter, Georg
dc.date.accessioned2022-11-18T09:50:33Z
dc.date.available2014-11-25T11:05:17Z
dc.date.available2022-11-18T09:50:33Z
dc.date.issued2014
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-112006
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9079
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8467
dc.description.abstractIn this study, we aimed to identify molecular mechanisms involved in the specification of the 4d (mesentoblast) lineage in Platynereis dumerilii. We employ RT-PCR and in situ hybridization against the Platynereis dumerilii twist homolog (Pdu-twist) to reveal mesodermal specification within this lineage. We show that Pdu-twist mRNA is already maternally distributed. After fertilization, ooplasmatic segregation leads to relocation of Pdu-twist transcripts into the somatoblast (2d) lineage and 4d, indicating that the maternal component of Pdu-twist might be an important prerequisite for further mesoderm specification but does not represent a defining characteristic of the mesentoblast. However, after the primordial germ cells have separated from the 4d lineage, zygotic transcription of Pdu-twist is exclusively observed in the myogenic progenitors, suggesting that mesodermal specification occurs after the 4d stage. Previous studies on spiral cleaving embryos revealed a spatio-temporal correlation between the 4d lineage and the activity of an embryonic organizer that is capable to induce the developmental fates of certain micromeres. This has raised the question if specification of the 4d lineage could be connected to the organizer activity. Therefore, we aimed to reveal the existence of such a proposed conserved organizer in Platynereis employing antibody staining against dpERK. In contrast to former observations in other spiralian embryos, activation of MAPK signaling during 2d and 4d formation cannot be detected which questions the existence of a conserved connection between organizer function and specification of the 4d lineage. However, our experiments unveil robust MAPK activation in the prospective nephroblasts as well as in the macromeres and some micromeres at the blastopore in gastrulating embryos. Inhibition of MAPK activation leads to larvae with a shortened body axis, defects in trunk muscle spreading and improper nervous system condensation, indicating a critical function for MAPK signaling for the reorganization of embryonic tissues during the gastrulation process.en
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subject.ddcddc:590de_DE
dc.titleMaternal inheritance of twist and analysis of MAPK activation in embryos of the polychaete Annelid Platynereis dumeriliien
dc.typearticlede_DE
local.affiliationFB 08 - Biologie und Chemiede_DE
local.opus.id11200
local.opus.instituteInstitut für Allgemeine und Spezielle Zoologiede_DE
local.opus.fachgebietBiologiede_DE
local.source.urihttps://doi.org/10.1371/journal.pone.0096702
local.source.freetextPLoS ONE 9(5):e96702de_DE


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