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dc.contributor.authorKosinska, Marta Krystyna
dc.contributor.authorLudwig, Taryn E.
dc.contributor.authorLiebisch, Gerhard
dc.contributor.authorZhang, Ruiyan
dc.contributor.authorSiebert, Hans-Christian
dc.contributor.authorWilhelm, Jochen
dc.contributor.authorKaesser, Ulrich
dc.contributor.authorDettmeyer, Reinhard B.
dc.contributor.authorKlein, Heiko
dc.contributor.authorIshaque, Bernd
dc.contributor.authorRickert, Markus
dc.contributor.authorSchmitz, Gerd
dc.contributor.authorSchmidt, Tannin A.
dc.contributor.authorSteinmeyer, Juergen
dc.date.accessioned2022-11-18T09:50:51Z
dc.date.available2016-01-29T10:06:57Z
dc.date.available2022-11-18T09:50:51Z
dc.date.issued2015
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-119030
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9146
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8534
dc.description.abstractBackground:Hyaluronic acid (HA), lubricin, and phospholipid species (PLs) contribute independently or together to the boundary lubrication of articular joints that is provided by synovial fluid (SF). Our study is the first reporting quantitative data about the molecular weight (MW) forms of HA, lubricin, and PLs in SF from cohorts of healthy donors, patients with early (eOA)- or late (lOA)-stage osteoarthritis (OA), and patients with active rheumatoid arthritis (RA). Methods: We used human SF from unaffected controls, eOA, lOA, and RA. HA and lubricin levels were measured by enzyme-linked immunosorbent assay. PLs was quantified by electrospray ionization tandem mass spectrometry. Fatty acids (FAs) were analyzed by gas chromatography, coupled with mass spectrometry. The MW distribution of HA was determined by agarose gel electrophoresis. Results: Compared with control SF, the concentrations of HA and lubricin were lower in OA and RA SF, whereas those of PLs were higher in OA and RA SF. Moreover, the MW distribution of HA shifted toward the lower ranges in OA and RA SF. We noted distinct alterations between cohorts in the relative distribution of PLs and the degree of FA saturation and chain lengths of FAs. Conclusions: The levels, composition, and MW distribution of all currently known lubricants in SF HA, lubricin, PLs vary with joint disease and stage of OA. Our study is the first delivering a comprehensive view about all joint lubricants during health and widespread joint diseases. Thus, we provide the framework to develop new optimal compounded lubricants to reduce joint destruction.en
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subject.ddcddc:610de_DE
dc.titleArticular joint lubricants during osteoarthritis and rheumatoid arthritis display altered levels and molecular speciesen
dc.typearticlede_DE
local.affiliationFB 11 - Medizinde_DE
local.opus.id11903
local.opus.instituteOrthopedic Research Laboratories, Department of Orthopedicsde_DE
local.opus.fachgebietMedizinde_DE
local.source.urihttps://doi.org/10.1371/journal.pone.0125192
local.source.freetextPLoS ONE 10(5):e0125192de_DE


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