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CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

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10.1038_srep31313.pdf (2.367Mb)
Date
2016
Author
Schneider, Tim
Hung, Lee-Hsueh
Schreiner, Silke
Starke, Stefan
Eckhof, Heinrich
Rossbach, Oliver
Reich, Stefan
Medenbach, Jan
Bindereif, Albrecht
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http://dx.doi.org/10.22029/jlupub-8637
Abstract

Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. ... By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-co-immunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component.

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https://doi.org/10.1038/srep31313
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