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dc.contributor.authorSchneider, Tim
dc.contributor.authorHung, Lee-Hsueh
dc.contributor.authorSchreiner, Silke
dc.contributor.authorStarke, Stefan
dc.contributor.authorEckhof, Heinrich
dc.contributor.authorRossbach, Oliver
dc.contributor.authorReich, Stefan
dc.contributor.authorMedenbach, Jan
dc.contributor.authorBindereif, Albrecht
dc.date.accessioned2022-11-18T09:51:33Z
dc.date.available2017-02-03T09:06:32Z
dc.date.available2022-11-18T09:51:33Z
dc.date.issued2016
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-124646
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9249
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8637
dc.description.abstractCircular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-co-immunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component.en
dc.language.isoende_DE
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddcddc:540de_DE
dc.titleCircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPsen
dc.typearticlede_DE
local.affiliationFB 08 - Biologie und Chemiede_DE
local.opus.id12464
local.opus.instituteInstitute of Biochemistryde_DE
local.opus.fachgebietBiochemie (FB 08)de_DE
local.source.urihttps://doi.org/10.1038/srep31313
local.source.freetextScientific Reports 6:31313de_DE


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