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Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV

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10.1186_s12917_016_0779_4.pdf (1.803Mb)
Date
2016
Author
Oellers, Dana E.
Bauer, Natali
Ginder, Melanie
Johannes, Sigrid
Pernecker, Iris
Moritz, Andreas
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http://dx.doi.org/10.22029/jlupub-8681
Abstract

Background: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30 38, ... 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate ( Oellers-gate ) and to establish reference intervals (RIs) in>120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. Results: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the Oellers-gate . Good correlation was found between the different gates (rs: 0.88 1.00). RIs for the Pankraz-gate , the Gelain-gate , and the Oellers-gate were 0.0 1.2, 0.2 3.7 and 0.2 3.9 % respectively. CVs were ranging between 22 and 41 %. Conclusions: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements.

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https://doi.org/10.1186/s12917-016-0779-4
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