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Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
| dc.contributor.author | Oellers, Dana E. | |
| dc.contributor.author | Bauer, Natali | |
| dc.contributor.author | Ginder, Melanie | |
| dc.contributor.author | Johannes, Sigrid | |
| dc.contributor.author | Pernecker, Iris | |
| dc.contributor.author | Moritz, Andreas | |
| dc.date.accessioned | 2022-11-18T09:51:55Z | |
| dc.date.available | 2017-05-26T12:56:08Z | |
| dc.date.available | 2022-11-18T09:51:55Z | |
| dc.date.issued | 2016 | |
| dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-128733 | |
| dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9293 | |
| dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8681 | |
| dc.description.abstract | Background: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30 38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate ( Oellers-gate ) and to establish reference intervals (RIs) in>120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. Results: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the Oellers-gate . Good correlation was found between the different gates (rs: 0.88 1.00). RIs for the Pankraz-gate , the Gelain-gate , and the Oellers-gate were 0.0 1.2, 0.2 3.7 and 0.2 3.9 % respectively. CVs were ranging between 22 and 41 %. Conclusions: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements. | en |
| dc.language.iso | en | de_DE |
| dc.rights | Namensnennung 4.0 International | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | RNA-rich platelets | en |
| dc.subject | Canine | en |
| dc.subject | Hematology analyzer | en |
| dc.subject.ddc | ddc:630 | de_DE |
| dc.title | Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV | en |
| dc.type | article | de_DE |
| local.affiliation | FB 10 - Veterinärmedizin | de_DE |
| local.opus.id | 12873 | |
| local.opus.institute | Department of Veterinary Clinical Sciences | de_DE |
| local.opus.fachgebiet | Veterinärmedizin | de_DE |
| local.source.uri | https://doi.org/10.1186/s12917-016-0779-4 | |
| local.source.freetext | BMC Veterinary Research 12:148 | de_DE |
