Human amniotic epithelial cells : Isolation and characterisation
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Abstract
The amniotic membrane is part of the fetal membrane and is composed of the amniotic epithelial (HAE) and mesenchymal (HAM) cells that are derived from the inner cell mass in the blastocyst. Thus, HAE and HAM cells may be multipotent. It has been reported that some differentiation markers for neuronal and hepatic parenchymal cells were expressed in HAE cells, suggesting that HAE cells may be a good source of stem cells for various cellular therapies. In addition, amniotic cells do not express the MHC class II, which may help prevent immune rejection, and can be obtained after delivery. We established a new method for isolation and cultivation of HAE cells and tested the pluripotency in different passages. HAE cells were cultured in different enriched mediums (with different growth factors) for expansion.We have found that HAE cells express Oct-4 mRNA as well as Rex-1 mRNAs (pluripotency markers) at passage 0. Phenotypic characterisation of HAE cells was carried out by a flow cytometer. HAE cells from passages 0, 1 and 5 demonstrated the transitive expression of epithelial, mesenchymal and endothelial markers.In summary, amnion from discarded placenta can be an interesting source of cells for regenerative medicine.Link to publications or other datasets
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Giessen : Laufersweiler