Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome

dc.contributor.authorTheuerkauf, Kim
dc.contributor.authorObach-Schröck, Carmen
dc.contributor.authorStaszyk, Carsten
dc.contributor.authorMoritz, Andreas
dc.contributor.authorRoscher, Katja A.
dc.date.accessioned2023-04-18T09:39:03Z
dc.date.available2023-04-18T09:39:03Z
dc.date.issued2022
dc.description.abstractIn humans, activated platelets contribute to sepsis complications and to multiple organ failure. In our prospective analytical study of cases of the equine systemic inflammatory response syndrome (SIRS), we adapted a standard human protocol for the measurement of activated platelets and platelet-leukocyte aggregates (PLAs) in equine platelet-leukocyte-rich plasma (PLRP) by flow cytometry, and we investigated the hypothesis that activated platelets and PLAs are increased in clinical cases of SIRS. We included 17 adult horses and ponies fulfilling at least 2 SIRS criteria, and 10 healthy equids as controls. Activation of platelets was determined by increased expression of CD62P on platelets. Activated platelets and PLAs were measured before and after in vitro activation of platelets with collagen. Median expression of CD62P on platelets was significantly increased after activation in the control group: 1.45% (interquartile range [IQR]: 1.08–1.99%) initially versus 8.78% (IQR: 6.79–14.78%, p = 0.002) after activation. The equids with SIRS had significantly more activated platelets and PLAs in native PLRP than controls: CD62P 4.92% (median, IQR: 2.21–12.41%) versus 1.45% in controls (median, IQR: 1.08–1.99%, p = 0.0007), and PLAs 4.16% (median, IQR: 2.50–8.58%) versus 2.95% in controls (median, IQR: 1.57–3.22%, p = 0.048). To our knowledge, increased platelet activation and PLAs have not been demonstrated previously with flow cytometry in clinical cases of equine SIRS.
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/16256
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-15639
dc.language.isoen
dc.rightsNamensnennung - Nicht kommerziell 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectCD62P
dc.subjectequine systemic inflammatory response syndrome
dc.subjectflow cytometry
dc.subjecthorses
dc.subjectP-selectin
dc.subjectplatelets
dc.subject.ddcddc:630
dc.titleActivated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome
dc.typearticle
local.affiliationFB 10 - Veterinärmedizin
local.source.epage457
local.source.journaltitleJournal of veterinary diagnostic investigation
local.source.number3
local.source.spage448
local.source.urihttps://doi.org/10.1177/10406387221077969
local.source.volume34

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