Hypoxia-induced gene expression in murine alveolar macrophages
dc.contributor.author | Israeli, Zeev | |
dc.date.accessioned | 2023-03-16T20:04:50Z | |
dc.date.available | 2009-02-24T10:41:15Z | |
dc.date.available | 2023-03-16T20:04:50Z | |
dc.date.issued | 2009 | |
dc.description.abstract | Hypoxia is known to trigger inflammatory reaction solely or in concert with other stimuli leading to lung damage. Hypoxia- induced lung damage is mediated by the secretion of pro-inflammatory cytokines by alveolar macrophages (AM). To learn more about the genes involved in the hypoxia mediated- activation of AM, m-RNA of AM from mice kept under normoxia/hypoxia for 1 or 21 days was isolated and studied using three established techniques: Microarrays, real-time PCR and immunohistochemistry. Array results confirmed that hypoxia is a mild stimulus for macrophages; No strongly regulated genes were identified and the expression pattern of selected genes as explored by PCR did not support arrays findings. Immunostaining for two selected genes demonstrated a decrease of vimentin expression under acute hypoxia (1 day) and an increase of integrin b2 expression under chronic hypoxia (21 days).Our findings show that hypoxia is a mild stimulus in AM; identification of genes with mild regulation is difficult so future studies should rely on a considerably larger sample sizes. Regulation was confirmed on protein level by immunohistochemical staining for two selected genes, more studies are needed for validation and better understanding of this phenomena. | en |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-68688 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/14015 | |
dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-13397 | |
dc.language.iso | en | de_DE |
dc.rights | In Copyright | * |
dc.rights.uri | http://rightsstatements.org/page/InC/1.0/ | * |
dc.subject | Hypoxia | en |
dc.subject | gene expresion | en |
dc.subject | alveolar macrophages | en |
dc.subject.ddc | ddc:610 | de_DE |
dc.title | Hypoxia-induced gene expression in murine alveolar macrophages | en |
dc.type | doctoralThesis | de_DE |
dcterms.dateAccepted | 2009-02-02 | |
local.affiliation | FB 11 - Medizin | de_DE |
local.opus.fachgebiet | Medizin | de_DE |
local.opus.id | 6868 | |
local.opus.institute | Institut für Pathologie | de_DE |
thesis.level | thesis.doctoral | de_DE |
Dateien
Originalbündel
1 - 1 von 1
Lade...
- Name:
- IsraeliZeev-2009-02-02.pdf
- Größe:
- 3.28 MB
- Format:
- Adobe Portable Document Format