A compact and mobile high resolution and high accuracy Mutliple-Reflection Time-of-Flight Mass Spectrometer (MR-TOF-MS) has been designed, built, commissioned and characterized. A new method delivers the capability for isobar separation and is a prerequisite for possible subsequent mass measurements (MS N) in future experiments. This work gives an introduction to the theoretical principles of high performance and in-situ mass spectrometry and describes the whole building chain of a powerful instrument - that has its origin in nuclear physics - and now features the application in in-situ analytics and in life science with high analytical value on a mobile platform.The wide field of mass spectrometry offers complex and stationary instruments with outstanding performance (mass resolving power Rm > 100000, mass accuracy delta m=m < 1 ppm) on one hand and portable, less powerful devices for in-situ investigation on the other hand. The concept of MR-TOF-MS offers high mass spectrometric performance in a compact and robust concept, offering high mass resolving power, high mass accuracy, short measurement cycles and lowest infrastructural needs. The MR-TOF-MS of this work has been designed with the goal to build a device, which combines the aspects of high performance and field deployment. It is capable of application in fundamental sciences for precision measurements, as well as for various life science analytics that can be performed directly at the investigation site. The mass resolving power of Rm > 300000 is independent on mass and enables the resolution of isobars and isotopes at even high molecular mass. The mass accuracy of delta m/m < 1 ppm allows to determine the composition and structure of biomolecules. Time-dependent studies such as chromatography and high throughput measurements are supported by a repetition rate of up to 2000 Hz. A new method for a highly mass-resolved separation with a resolution of Rs > 60000 may enable distinct precursor selection for tandem experiments and fragmentation studies in future, as well as the removal of isobaric contaminants in nuclear physics experiments.The mass spectrometer itself relies on an effective combination of radio frequency quadrupoles and a multiple-reflection time-of-flight analyzer. Ion optics and system supply have been shaped into a rugged and transportable format. An atmospheric pressure interface offers great flexibility in the choice of the ion source and provides an essential in-situ aspect. Most components are custom-made and specially developed. This prototype instrument is ruggedized, reliable, stable in operation with a start-up time of 20 min only and therefore ideally suited for in-situ application like in the field of medicine, civil safety and environmental research. There is no need for further infrastructure.
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