Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent

dc.contributor.authorMunoz-Caro, Tamara
dc.contributor.authorMena Huertas, Sandra Jaqueline
dc.contributor.authorConejeros, Ivan
dc.contributor.authorAlarcon, Pablo
dc.contributor.authorHidalgo, Maria A
dc.contributor.authorBurgos, Rafael A
dc.contributor.authorHermosilla, Carlos
dc.contributor.authorTaubert, Anja
dc.date.accessioned2022-11-18T09:50:49Z
dc.date.available2015-10-01T13:06:42Z
dc.date.available2022-11-18T09:50:49Z
dc.date.issued2015
dc.description.abstractEimeria bovis is an important coccidian parasite that causes high economic losses in the cattle industry. We recently showed that polymorphonuclear neutrophils (PMN) react upon E. bovis sporozoite exposure by neutrophil extracellular trap (NET) formation. We focused here on the molecular mechanisms that are involved in this process. The sporozoite encounter led to an enhanced surface expression of neutrophil CD11b suggesting a potential role of this receptor in E. bovis-mediated NETosis. Antibody-mediated blockage of CD11b confirmed this assumption and led to a significantly decreased sporozoite-triggered NET. In addition, E. bovis-induced NETosis was found to be Ca2+-dependent since the inhibition of store-operated calcium entry (SOCE) significantly diminished NET. Furthermore, NADPH oxidase, neutrophil elastase (NE) and myeloperoxidase (MPO) were confirmed as key molecules in sporozoite-triggered NETosis, as inhibition thereof blocked parasite-triggered NET. PMN degranulation analyses revealed a significant release of matrix metalloprotease-9 containing granules upon sporozoite exposure. We further show a significantly enhanced phosphorylation of ERK1/2 and p38 MAPK in sporozoite-exposed PMN indicating a key role of this signaling pathway in E. bovis-mediated NETosis. Accordingly, ERK 1/2 and p38 MAPK inhibition led to a significant decrease in NET formation. Finally, we demonstrate that sporozoite-induced NETosis is neither a stage-, species-, nor host-specific process.en
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-117214
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9139
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8527
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subject.ddcddc:610de_DE
dc.titleEimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependenten
dc.typearticlede_DE
local.affiliationFB 10 - Veterinärmedizinde_DE
local.opus.fachgebietVeterinärmedizinde_DE
local.opus.id11721
local.opus.instituteInstitute of Parasitologyde_DE
local.source.freetextVeterinary Research 46(1):23de_DE
local.source.urihttps://doi.org/10.1186/s13567-015-0155-6

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