Prevention of demineralisation by addition of chlorhexidine in the adhesive procedure of composite resins assessed with an artificial mouth model
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DOI:
http://dx.doi.org/10.22029/jlupub-14687Abstract
ObjectiveAim of the study was to investigate the antibacterial and antiproteolytic effect of chlorhexidine (CHX) when added in the adhesive procedure, at baseline, after biological loading and after 6- or 12-month storage in distilled water or in artificial saliva.Materials & MethodsThe study consisted of two parts: biological loading of restorations in a bacterial caries model (n=60 teeth) and μTBS at baseline, after biological loading and after storage (n=2979 sticks). Following groups were formed: i) Control group (CTRL), 3-step adhesive Adper Scotchbond Multipurpose, 3M ESPE, ii) 2% CHX as dentine pre-treatment (DENT), iii) 0.1% CHX in primer (PRIM), iv) 0.1% CHX in bonding (BOND), v) 2-step adhesive Peak® Universal Bond with 0.2% CHX (PEAK). PRIM and BOND were fabricated on the basis of the 3-step adhesive used. Class V composite restorations were loaded in caries model with S. mutans according to a 10-day biological protocol. Demineralisation was evaluated with fluorescence microscopy and marginal analysis was performed with SEM. For μTBS (Syndicad TC-550), dentine-composite sticks with 0.46 mm2 adhesive interface were fabricated and tested at baseline, after 2-day caries model, after 6- and 12-month storage in distilled water or artificial saliva. Fracture mode analysis followed and exemplary sticks were evaluated under SEM. ResultsNone of the CHX adhesives inhibited secondary caries formation but 2% CHX dentine pre-treatment (DENT) managed to limit marginal gap formation in enamel (p<0.05). Bond strength of CHX adhesives at baseline was worse compared to the CTRL (p<0.05) and decreased significantly after caries model (p<0.05). Only PRIM exhibited same μTBS values after 12-month storage in saliva, compared to baseline. Conclusions2% as dentine pre-treatment inhibited marginal degradation at enamel margins, but no CHX adhesive protected composite restorations against secondary caries. 0.1% CHX in primer protected bond strength degradation after 12 months in artificial saliva, exhibiting a potential antiproteolytic effect.Link to publications or other datasets
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Giessen : VVB Laufersweiler Verlag