The hepatic response following infection with Listeria monocytogenes
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Abstract
In the present study, we investigated the liver specific response upon infection with L. monocytogenes, a model pathogen for Gram-positive infections over a period of 5days. We used whole genome microarray chips to determine the temporal transcriptome at five observation points. Relative mRNA levels were validated for a representative subset of genes by quantitative Real-Time PCR. In the analysis of these data we followed a strict methodology. The quality of microarray data was ensured by several measures, including quality control tools developed and optimized at our institution. The biological effects of differentially expressed genes were investigated and interpretation of these results was followed by confirmatory experiments.In conclusion, this work allows a unique insight into regulatory networks of several biological processes and interconnections following an infection with L. monocytogenes. Based on our results and by integration of known literature, LXR-a and related transcription factors are proposed to be fundamental for the regulation of hepatic and subsequently systemic response to pathogens.Link to publications or other datasets
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Giessen : VVB Laufersweiler