Analysis of organochlorines and polycyclic aromatic hydrocarbons designed for pollutant biomonitoring in three seabird matrices

Abstract

Pollutant biomonitoring demands analytical methods to cover a wide range of target compounds, work with minimal sample amounts, and apply least invasive and reproducible sampling procedures. We developed a method to analyse 68 bioaccumulative organic pollutants in three seabird matrices: plasma, liver, and stomach oil, representing different exposure phases. Extraction efficiency was assessed based on recoveries of spiked surrogate samples, then the method was applied to environmental samples collected from Scopoli’s shearwater (Calonectris diomedea). Extraction was performed in an ultrasonic bath, purification with Florisil cartridges (5 g, 20 mL), and analysis by GC–Orbitrap–MS. Quality controls at 5 ng yielded satisfactory recoveries (80–120%) although signal intensification was found for some compounds. The method permitted the detection of 28 targeted pollutants in the environmental samples. The mean sum of organic pollutants was 4.25 ± 4.83 ng/g in plasma, 1634 ± 2990 ng/g in liver, and 233 ± 111 ng/g in stomach oil (all wet weight). Pollutant profiles varied among the matrices, although 4,4′-DDE was the dominant compound overall. This method is useful for pollutant biomonitoring in seabirds and discusses the interest of analysing different matrices.