Aphid gel saliva : Sheath structure, protein composition and secretory dependence on stylet-tip milieu

dc.contributor.authorWill, Torsten
dc.contributor.authorSteckbauer, Kathrin
dc.contributor.authorHardt, Martin
dc.contributor.authorvan Bel, Aart J. E.
dc.date.accessioned2022-11-18T09:56:55Z
dc.date.available2012-11-20T14:18:32Z
dc.date.available2022-11-18T09:56:55Z
dc.date.issued2012
dc.description.abstractIn order to separate and analyze saliva types secreted during stylet propagation and feeding, aphids were fed on artificial diets. Gel saliva was deposited as chains of droplets onto Parafilm membranes covering the diets into which watery saliva was secreted. Saliva compounds collected from the diet fluid were separated by SDS-PAGE, while non-soluble gel saliva deposits were processed in a novel manner prior to protein separation by SDS-PAGE. Soluble (watery saliva) and non-soluble (gel saliva) protein fractions were significantly different. To test the effect of the stylet milieu on saliva secretion, aphids were fed on various diets. Hardening of gel saliva is strongly oxygen-dependent, probably owing to formation of sulfide bridges by oxidation of sulphydryl groups. Surface texture of gel saliva deposits is less pronounced under low-oxygen conditions and disappears in dithiothreitol containing diet. Using diets mimicking sieve-element sap and cell-wall fluid respectively showed that the soluble protein fraction was almost exclusively secreted in sieve elements while non-soluble fraction was preferentially secreted at cell wall conditions. This indicates that aphids are able to adapt salivary secretion in dependence of the stylet milieu.en
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-90731
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9662
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-9050
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subjectaphidsen
dc.subjectsaliva secretionen
dc.subjectwatery / gel salivaen
dc.subjectstylet milieuen
dc.subjectoxygen dependenceen
dc.subject.ddcddc:570de_DE
dc.titleAphid gel saliva : Sheath structure, protein composition and secretory dependence on stylet-tip milieuen
dc.typearticlede_DE
local.affiliationZentrende_DE
local.opus.fachgebietZentrale Biotechnische Betriebseinheitde_DE
local.opus.id9073
local.opus.institutePlant Cell Biology Research Groupde_DE
local.source.freetextPLoS ONE, 7(10), e46903, 1-8de_DE
local.source.urihttps://doi.org/10.1371/journal.pone.0046903

Dateien

Originalbündel
Gerade angezeigt 1 - 1 von 1
Lade...
Vorschaubild
Name:
journal.pone.0046903.pdf
Größe:
610.87 KB
Format:
Adobe Portable Document Format