Rubisco : easy purification and immunochemical determination

dc.contributor.authorGroß, Ulrich
dc.date.accessioned2023-06-12T07:53:46Z
dc.date.available2003-09-23T08:06:55Z
dc.date.available2023-06-12T07:53:46Z
dc.date.issued2001
dc.description.abstractRubisco (Ribulose-1.5-bisphosphate carboxylase/oxygenase) from spinach was purified to homogeneity in one step by gel filtration. This enzyme is suitable for the generation of a specific antibody in rabbits. The enzyme concentration in spinach leaves amounted to 40 % of the total soluble protein. The specific antibody shows cross reaction with crude extracts from leaves of other higher plants. The enzymes subunits could be separated by denaturing preparative SDS gel electrophoresis.en
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-12426
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/16690
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-16068
dc.language.isoende_DE
dc.relation.ispartofhttps://doi.org/10.22029/jlupub-17634
dc.rightsIn Copyright*
dc.rights.urihttp://rightsstatements.org/page/InC/1.0/*
dc.subject.ddcddc:570de_DE
dc.titleRubisco : easy purification and immunochemical determinationen
dc.typebookPartde_DE
local.affiliationFB 09 - Agrarwissenschaften, Ökotrophologie und Umweltmanagementde_DE
local.opus.fachgebietHaushalts- und Ernährungswissenschaften - Ökotrophologiede_DE
local.opus.id1242
local.opus.instituteInstitute of Plant Nutrition, Department of Tissue Culture, Justus-Liebig-Universität Giessende_DE
local.source.freetextBender, L. und Kumar, A. (Eds.): From soil to cell. Giessen 2001

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