Evaluation of canine and feline leukocyte differential counts obtained with the scil vCell 5 compared to the Advia 2120 hematology analyzer and a manual method

dc.contributor.authorZelmer, Kim-Lina Charlotte
dc.contributor.authorMoritz, Andreas
dc.contributor.authorBauer, Natali
dc.date.accessioned2024-10-01T13:03:27Z
dc.date.available2024-10-01T13:03:27Z
dc.date.issued2023
dc.description.abstractThe vCell 5 (scil Animal Care), a point-of-care hematology analyzer (POCA), was recently introduced to veterinary laboratories. This laser- and impedance-based analyzer is capable of providing a CBC with 5-part WBC differential count (Diff) along with WBC cytograms and flags serving as interpretation aids for numerical results. We compared the scil POCA-Diff to reference methods (i.e., manual differential count, Advia 2120 hematology analyzer [Siemens]) for canine and feline blood samples and considered WBC cytograms and flags. Total observed error (TEo), calculated from CV and bias%, was compared to total allowable error (TEa). Data were analyzed before and after a review process (exclusion of flagged and samples with invalid cytograms). For both species, correlation was good-to-excellent (rs = 0.81–0.97) between both analyzers for all variables, except for feline monocytes (rs = 0.21–0.63) and canine monocyte% (rs = 0.50). Smallest biases were seen for neutrophils (dog: −5.7 to 0.8%; cat: 1.5–9.4%) with both reference methods. Quality requirements (TEo < TEa) were fulfilled for canine and feline neutrophils (TEo = 5.3–10.6%, TEa = 15%) and eosinophils (TEo = 67.1–83%, TEa = (90)–50%) considering at least one reference method. Our review process led to mildly higher rs-values for most variables. Although not completely satisfactory, the scil POCA provides reliable results in compliance with ASVCP quality goals for canine and feline neutrophils and eosinophils. Analyzer flag and cytogram analysis served as useful tools for QA, indicating the necessity for manual review of blood smears, and contributed to improvement of scil POCA performance.en
dc.identifier.urihttps://jlupub.ub.uni-giessen.de/handle/jlupub/19543
dc.identifier.urihttps://doi.org/10.22029/jlupub-18901
dc.language.isoen
dc.rightsNamensnennung - Nicht kommerziell 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddcddc:630
dc.titleEvaluation of canine and feline leukocyte differential counts obtained with the scil vCell 5 compared to the Advia 2120 hematology analyzer and a manual method
dc.typearticle
local.affiliationFB 10 - Veterinärmedizin
local.source.epage697
local.source.journaltitleJournal of veterinary diagnostic investigation
local.source.spage679
local.source.urihttps://doi.org/10.1177/10406387231187899
local.source.volume35

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