In situ hybridization of the feline major satellite DNA FA-SAT in feline fibrosarcoma cell lines and feline fibrosarcoma tissue sections
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Abstract
Feline fibrosarcomas are the most common skin tumors of cats. Despite this high frequency and the publication of different hypotheses for their pathogenesis by several authors, the alterations accompanying the development of this tumor are still not completely understood.We studied the feline major satellite DNA (FA-SAT) hybridization pattern by FISH in four fibrosarcoma cell lines and one normal embryonic fibroblastic cell line as well as in 30 fibrosarcomas from 28 cats. Of the latter, tumor tissue biopsies and biopsies of non-affected skin of the same patient were analyzed.The hybridization pattern of the FA-SAT in the normal fibroblastic cell line differed significantly from that observed in all fibrosarcoma cell lines. The same observation was made for tumor tissues and fibroblasts from non-affected skin in paraffin-embedded material. These differences observed in vitro and in vivo suggest that alterations of the FA-SAT may be associated with chromosomal instability in the feline fibrosarcoma.Additionally, our FISH results show the usability of the designed method in differentiating tumor cells from normal skin fibroblasts in feline fibrosarcoma.Link to publications or other datasets
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