A high-resolution map of functional miR-181 response elements in the thymus reveals the role of coding sequence targeting and an alternative seed match
dc.contributor.author | Verheyden, Nikita A | |
dc.contributor.author | Klostermann, Melina | |
dc.contributor.author | Brüggemann, Mirko | |
dc.contributor.author | Steede, Hanna M | |
dc.contributor.author | Scholz, Anica | |
dc.contributor.author | Amr, Shady | |
dc.contributor.author | Lichtenthaeler, Chiara | |
dc.contributor.author | Münch, Christian | |
dc.contributor.author | Schmid, Tobias | |
dc.contributor.author | Zarnack, Kathi | |
dc.contributor.author | Krueger, Andreas | |
dc.date.accessioned | 2024-11-01T14:20:43Z | |
dc.date.available | 2024-11-01T14:20:43Z | |
dc.date.issued | 2024 | |
dc.description.abstract | MicroRNAs (miRNAs) are critical post-transcriptional regulators in many biological processes. They act by guiding RNA-induced silencing complexes to miRNA response elements (MREs) in target mRNAs, inducing translational inhibition and/or mRNA degradation. Functional MREs are expected to predominantly occur in the 3′ untranslated region and involve perfect base-pairing of the miRNA seed. Here, we generate a high-resolution map of miR-181a/b-1 (miR-181) MREs to define the targeting rules of miR-181 in developing murine T cells. By combining a multi-omics approach with computational high-resolution analyses, we uncover novel miR-181 targets and demonstrate that miR-181 acts predominantly through RNA destabilization. Importantly, we discover an alternative seed match and identify a distinct set of targets with repeat elements in the coding sequence which are targeted by miR-181 and mediate translational inhibition. In conclusion, deep profiling of MREs in primary cells is critical to expand physiologically relevant targetomes and establish context-dependent miRNA targeting rules. | en |
dc.description.sponsorship | Deutsche Forschungsgemeinschaft (DFG); ROR-ID:018mejw64 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de/handle/jlupub/19748 | |
dc.identifier.uri | https://doi.org/10.22029/jlupub-19105 | |
dc.language.iso | en | |
dc.rights | Namensnennung 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.ddc | ddc:570 | |
dc.title | A high-resolution map of functional miR-181 response elements in the thymus reveals the role of coding sequence targeting and an alternative seed match | |
dc.type | article | |
local.affiliation | FB 08 - Biologie und Chemie | |
local.project | SFB 902-B15, KR 2320/7-1, GRK 2355/2, SFB 902-B13, FOR 2333-TP2 | |
local.source.articlenumber | gkae416 | |
local.source.epage | 19 | |
local.source.journaltitle | Nucleic acids research | |
local.source.spage | 1 | |
local.source.uri | https://doi.org/10.1093/nar/gkae416 | |
local.source.volume | 116 |
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