Hypoxia-inducible factor-1alpha activation in HPV-positive head and neck squamous cell carcinoma cell lines
dc.contributor.author | Knuth, Jennifer | |
dc.contributor.author | Sharma, Shachi J. | |
dc.contributor.author | Würdemann, Nora | |
dc.contributor.author | Holler, Claudia | |
dc.contributor.author | Garvalov, Boyan K. | |
dc.contributor.author | Acker, Till | |
dc.contributor.author | Wittekindt, Claus | |
dc.contributor.author | Wagner, Steffen | |
dc.contributor.author | Klussmann, Jens P. | |
dc.date.accessioned | 2022-11-18T09:55:56Z | |
dc.date.available | 2020-12-01T15:03:11Z | |
dc.date.available | 2022-11-18T09:55:56Z | |
dc.date.issued | 2017 | |
dc.description.abstract | Purpose: Human papillomavirus (HPV) is a causative agent for a rising number of head and neck squamous cell carcinomas (HNSCC), which are characterized by distinct tumor biology. Hypoxia inducible-factor (HIF) signaling influences initiation and progression of carcinogenesis and HPV oncoproteins have evolved to highjack cellular pathways for viral reproduction. Therefore, we investigated whether HPV activates HIF-1alpha expression in HNSCC.Experimental Technique: HPV-positive and -negative HNSCC cells were examined for adaptive responses to hypoxia. Expression of HIF-1alpha, prolyl hydroxylasedomain protein 2 (PHD2) and E-cadherin was analyzed by Western blotting, immunofluorescence (IF) microscopy and migration/wound healing assays.Results: HPV-positive HNSCC cells showed higher HIF-1alpha and PHD2 protein levels under normoxia and hypoxia. HIF-1alpha hydroxylation was reduced in HPV-positive HNSCC cell lines under PHD and proteasomal inhibition. In vitro wound healing assays showed impairment of migration and proliferation by HIF-1alpha pathway activation in HPV-negative cell lines only. In contrast, migration and proliferation in HPV-positive cell lines was impaired by HIF-1alpha specific siRNA.Conclusions: HPV-positive HNSCC cells show activation of the HIF pathway and adaptation to HIF-1alpha upregulation, representing potential therapeutic targets in this emerging tumor entity. | en |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-157532 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9605 | |
dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8993 | |
dc.language.iso | en | de_DE |
dc.rights | Namensnennung 3.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | * |
dc.subject | human papillomavirus (hpv) | en |
dc.subject | head and neck squamous cell carcinoma (hnscc) | en |
dc.subject | hypoxia | en |
dc.subject | hypoxia inducible factor-1alpa (hif-1alpha) | en |
dc.subject.ddc | ddc:610 | de_DE |
dc.title | Hypoxia-inducible factor-1alpha activation in HPV-positive head and neck squamous cell carcinoma cell lines | en |
dc.type | article | de_DE |
local.affiliation | FB 11 - Medizin | de_DE |
local.opus.fachgebiet | Medizin | de_DE |
local.opus.id | 15753 | |
local.opus.institute | Department of Otorhinolaryngology, Head and Neck Surgery | de_DE |
local.source.freetext | Oncotarget 8:89681-89691 | de_DE |
local.source.uri | https://doi.org/10.18632/oncotarget.20813 |
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