The thesis aimed to investigate the expression and interaction profile of Protein tyrosine phosphatase interacting protein 51 (PTPIP51) in adipose tissue. Several groups of mice were trained and fed different diets to obtain different levels of insulin resistance. The training was held at 80% of maximum oxygen consumption, as an indicator for aerobe endurance training. The glucose tolerance of each mice was determined at the end of the test period. The expression profile of PTPIP51 in adipose tissue was investigated by immunofluorescence, PCR and Duolink proximity ligation assay.PTPIP51 was detected in all investigated samples. Its interaction partners PTP1B,14-3-3ß, Insulin receptor (IR) and PKA also were expressed throughout all investigated groups.The interaction profile of PTPIP51 with its interaction partners differed widely between the groups. PTPIP51 interacts with PTP1B in trained high fat diet animals, normal diet animals and, to a lower extent, high fat diet animals. Interactions between PTPIP51 and 14-3-3ß was highest in trained high fat diet animals, whereas it was lowest in high fat diet animals. IR-PTPIP51 interaction was highest in high fat diet animals and lowest in trained standard diet animals. Normal diet and trained high fat diet animals did not show significant differences. The interaction between PTPIP51 and PKA was low in trained standard diet animals, all other groups did not differ significantly in their interaction profile.In conclusion, this thesis demonstrates the involvement of PTPIP51 in metabolic pathways. The protein seems to act as a switcher between the essential regulatory pathways for lipogenesis and lipolysis in adipocytes.
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