Validation of the 13C-sodium-acetate breath test for the measurement of gastric emptying in dogs in comparison to 99mTechnetium radioscintigraphy
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The gold standard method to assess gastric emptying in both humans and animals has been suggested to be 99mT scintigraphy. Lately, non-invasive 13C-breath tests have been used successfully as an alternative in human medicine, but have not been compared to scintigraphy in the dog. The aim of this study was to evaluate the 13C-sodium acetate breath test and compare it to gastric scintigraphy for a solid test meal in the dog. 12 privately owned healthy dogs were included. Age and weight ranged from 1.5 to 12 years and 9.2 to 38.1 kg, respectively. Normal liver function was ascertained in all dogs via bile acid stimulation test. Test meal consisted of canned dog food; the caloric intake was calculated for each dog based on body mass. The meal, which was labelled with either 100 mg 13C-sodium acetate or 150-250 MBq 99mTc colloid, was fed after an overnight fast and 13C-sodium acetate breath test and scintigraphy were performed on two consecutive days; this set of procedure was repeated in all dogs in an alternating order. Breath samples and scintigrams were obtained at time point 0 and every 15 minutes after the ingestion of the labelled food for 4 hours, then every 30 minutes for another 2 hours. 12CO2/13CO2 ratio in the breath was measured by non-dispersive infrared spectrometry. Gastric half emptying times for breath test analysis (Gt½b) and scintigraphy (Gt½s) were calculated for both sets of measurement, and compared with non-parametric statistic tests, as well as to compare scintigraphy and breath test data. The median Gt½b was 121 and 115 minutes (range 32 to 312 min) and the median Gt½s was 105 and 149 minutes (range 24 to 220 min). Gt½b and Gt½s did not correlate in the first set of measurements, but showed significant correlation in the second set (p< 0.05). Neither Gt½b nor Gt½s correlated between both sets of measurements. The 13C-sodium-acetate breath test is well tolerated in dogs and the test is easily performed. Since there was no correlation between both sets of scintigraphic measurements, it is questionable if this type of gastric emptying assessment can indeed be considered a gold standard. Large individual variations of gastric emptying times in a small cohort of dogs likely explain why the breath test results did not correlate but the ease of performance renders this test interesting for future studies.Verknüpfung zu Publikationen oder weiteren Datensätzen
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Giessen : VVB Laufersweiler 2007