Micro-lightguide spectrophotometry assessment of hepatic and intestinal microcirculation in endotoxemic rats during intravenous treatment with angiotensin II
dc.contributor.author | Schmidt, Götz | |
dc.contributor.author | Pitz, Laurenz | |
dc.contributor.author | Markmann, Melanie | |
dc.contributor.author | Schneck, Emmanuel | |
dc.contributor.author | Sander, Michael | |
dc.contributor.author | Koch, Christian | |
dc.contributor.author | Edinger, Fabian | |
dc.date.accessioned | 2024-10-01T13:06:26Z | |
dc.date.available | 2024-10-01T13:06:26Z | |
dc.date.issued | 2023 | |
dc.description.abstract | Introduction: During septic shock, impairment of microcirculation leads to enhanced permeability of intestinal mucosa triggered by generalized vasodilation and capillary leak. Intravenous angiotensin II (AT-II) has been approved for the treatment of septic shock; however, no in-vivo data exist on the influence of AT-II on hepatic and intestinal microcirculation. Material and methods: Sixty male Lewis rats were randomly assigned to six study groups (each n = 10): sham, lipopolysaccharide-induced septic shock, therapy with low- or high-dose AT-II (50 or 100 ng/kg/min, respectively), and septic shock treated with low- or high-dose AT-II. After median laparotomy, hepatic and intestinal microcirculation measures derived from micro-lightguide spectrophotometry were assessed for 3 h and included oxygen saturation (SO2), relative blood flow (relBF) and relative hemoglobin level (relHb). Hemodynamic measurements were performed using a left ventricular conductance catheter, and blood samples were taken hourly to analyze blood gasses and systemic cytokines. Results: AT-II increased mean arterial pressure in a dose-dependent manner in both septic and non-septic animals (p < 0.001). Lower hepatic and intestinal SO2 (both p < 0.001) were measured in animals without endotoxemia who received high-dose AT-II treatment, however, significantly impaired cardiac output was also reported in this group (p < 0.001). In endotoxemic rats, hepatic relBF and relHb were comparable among the treatment groups; however, hepatic SO2 was reduced during low- and high-dose AT-II treatment (p < 0.001). In contrast, intestinal SO2 remained unchanged despite treatment with AT-II. Intestinal relBF (p = 0.028) and interleukin (IL)-10 plasma levels (p < 0.001) were significantly elevated during treatment with high-dose AT-II compared with low-dose AT-II. Conclusions: A dose-dependent decrease of hepatic and intestinal microcirculation during therapy with AT-II in non-septic rats was observed, which might have been influenced by a corresponding reduction in cardiac output due to elevated afterload. While hepatic microcirculation was reduced during endotoxemia, no evidence for a reduction in intestinal microcirculation facilitated by AT-II was found. In contrast, both intestinal relBF and anti-inflammatory IL-10 levels were increased during high-dose AT-II treatment. | en |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de/handle/jlupub/19544 | |
dc.identifier.uri | https://doi.org/10.22029/jlupub-18902 | |
dc.language.iso | en | |
dc.rights | Namensnennung 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.ddc | ddc:610 | |
dc.title | Micro-lightguide spectrophotometry assessment of hepatic and intestinal microcirculation in endotoxemic rats during intravenous treatment with angiotensin II | |
dc.type | article | |
local.affiliation | FB 11 - Medizin | |
local.source.articlenumber | 106588 | |
local.source.epage | 12 | |
local.source.journaltitle | European journal of pharmaceutical sciences | |
local.source.spage | 1 | |
local.source.uri | https://doi.org/10.1016/j.ejps.2023.106588 | |
local.source.volume | 191 |
Dateien
Originalbündel
1 - 1 von 1
Lade...
- Name:
- 10.1016_j.ejps.2023.106588.pdf
- Größe:
- 3.04 MB
- Format:
- Adobe Portable Document Format