The Functional Importance of CD177 on Neutrophil in Interaction with Endothelium
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Abstract
This study sheds light on the characterisation of CD177 and identification of CD177s counter receptor on endothelium. We could demonstrate that PECAM-1 on endothelial cells functions directly as the counter receptor for CD177 by following observations; 1. Our recombinant CD177-Fc fusion protein, which was produced in insect cells, reacted with 130 KDa molecules on endothelium. 2. Screening with different mabs identified the 130 KDa protein as endothelial PECAM-1. 3. Biochemical and immunological studies using recombinant CD177-Fc fusion protein confirmed that endothelial PECAM-1 functions directly as the counter-receptor for CD177. 4. Functional studies in vitro demonstrated that purified CD177 protein inhibits CD177 mediated cell adhesion to immobilized PECAM-1 protein as well as neutrophil migration through endothelial cells. These results indicate that CD177 can act as cell adhesion molecule involving neutrophil-endothelial interaction. This observation may provide a new concept in understanding the multi-step process of transendothelial migration.In the future, this concept should be studied in different in vitro as well as in vivo model in more detail.Link to publications or other datasets
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Giessen : VVB Laufersweiler 2007