Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3
dc.contributor.author | Schneider, Tim | |
dc.contributor.author | Hung, Lee-Hsueh | |
dc.contributor.author | Aziz, Masood | |
dc.contributor.author | Wilmen, Anna | |
dc.contributor.author | Thaum, Stephanie | |
dc.contributor.author | Wagner, Jaqueline | |
dc.contributor.author | Janowski, Robert | |
dc.contributor.author | Müller, Simon | |
dc.contributor.author | Schreiner, Silke | |
dc.contributor.author | Friedhoff, Peter | |
dc.contributor.author | Hüttelmaier, Stefan | |
dc.contributor.author | Niessing, Dierk | |
dc.contributor.author | Sattler, Michael | |
dc.contributor.author | Schlundt, Andreas | |
dc.contributor.author | Bindereif, Albrecht | |
dc.date.accessioned | 2022-11-18T09:54:20Z | |
dc.date.available | 2019-10-22T06:49:11Z | |
dc.date.available | 2022-11-18T09:54:20Z | |
dc.date.issued | 2019 | |
dc.description.abstract | How multidomain RNA-binding proteins recognize their specific target sequences, based on a combinatorial code, represents a fundamental unsolved question and has not been studied systematically so far. Here we focus on a prototypical multidomain RNA-binding protein, IMP3 (also called IGF2BP3), which contains six RNA-binding domains (RBDs): four KH and two RRM domains. We establish an integrative systematic strategy, combining single-domain-resolved SELEX-seq, motif-spacing analyses, in vivo iCLIP, functional validation assays, and structural biology. This approach identifies the RNA-binding specificity and RNP topology of IMP3, involving all six RBDs and a cluster of up to five distinct and appropriately spaced CA-rich and GGC-core RNA elements, covering a >100 nucleotide-long target RNA region. Our generally applicable approach explains both specificity and flexibility of IMP3-RNA recognition, allows the prediction of IMP3 targets, and provides a paradigm for the function of multivalent interactions with multidomain RNA-binding proteins in gene regulation. | en |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-148931 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9501 | |
dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8889 | |
dc.language.iso | en | de_DE |
dc.rights | Namensnennung 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.ddc | ddc:540 | de_DE |
dc.title | Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3 | en |
dc.type | article | de_DE |
local.affiliation | FB 08 - Biologie und Chemie | de_DE |
local.opus.fachgebiet | Chemie | de_DE |
local.opus.id | 14893 | |
local.opus.institute | Institute of Biochemistry | de_DE |
local.source.freetext | Nature Communications 10:2266 | de_DE |
local.source.uri | https://doi.org/10.1038/s41467-019-09769-8 |
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