High-resolution atmospheric-pressure MALDI mass spectrometry imaging workflow for lipidomic analysis of late fetal mouse lungs

dc.contributor.authorGarikapati, Vannuruswamy
dc.contributor.authorKarnati, Srikanth
dc.contributor.authorBhandari, Dhaka Ram
dc.contributor.authorBaumgart-Vogt, Eveline
dc.contributor.authorSpengler, Bernhard
dc.date.accessioned2022-11-18T09:54:25Z
dc.date.available2019-10-22T10:16:52Z
dc.date.available2022-11-18T09:54:25Z
dc.date.issued2019
dc.description.abstractMass spectrometry imaging (MSI) provides label-free, non-targeted molecular and spatial information of the biomolecules within tissue. Lipids play important roles in lung biology, e.g. as surfactant, preventing alveolar collapse during normal and forced respiration. Lipidomic characterization of late fetal mouse lungs at day 19 of gestation (E19) has not been performed yet. In this study we employed high-resolution atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization MSI for the lipidomic analysis of E19 mouse lungs. Molecular species of different lipid classes were imaged in E19 lung sections at high spatial and mass resolution in positive- and negative-ion mode. Lipid species were characterized based on accurate mass and on-tissue tandem mass spectrometry. In addition, a dedicated sample preparation protocol, homogenous deposition of matrices on tissue surfaces and data processing parameters were optimized for the comparison of signal intensities of lipids between different tissue sections of E19 lungs of wild type and Pex11beta-knockout mice. Our study provides lipid information of E19 mouse lungs, optimized experimental and data processing strategies for the direct comparison of signal intensities of metabolites (lipids) among the tissue sections from MSI experiments. To best of our knowledge, this is the first MSI and lipidomic study of E19 mouse lungs.en
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-149001
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9507
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-8895
dc.language.isoende_DE
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddcddc:540de_DE
dc.titleHigh-resolution atmospheric-pressure MALDI mass spectrometry imaging workflow for lipidomic analysis of late fetal mouse lungsen
dc.typearticlede_DE
local.affiliationFB 08 - Biologie und Chemiede_DE
local.opus.fachgebietChemiede_DE
local.opus.id14900
local.opus.instituteInstitute of Inorganic and Analytical Chemistryde_DE
local.source.freetextScientific Reports 9:3192de_DE
local.source.urihttps://doi.org/10.1038/s41598-019-39452-3

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