Supplementation of carnitine leads to an activation of the IGF-1/PI3K/Akt signalling pathway and down regulates the E3 ligase MuRF1 in skeletal muscle of rats
dc.contributor.author | Keller, Janine | |
dc.contributor.author | Couturier, Aline | |
dc.contributor.author | Haferkamp, Melanie | |
dc.contributor.author | Most, Erika | |
dc.contributor.author | Eder, Klaus | |
dc.date.accessioned | 2022-11-18T09:50:21Z | |
dc.date.available | 2013-08-20T09:03:34Z | |
dc.date.available | 2022-11-18T09:50:21Z | |
dc.date.issued | 2013 | |
dc.description.abstract | BACKGROUND:Recently, it has been shown that carnitine down-regulates genes involved in the ubiquitin-proteasome system (UPS) in muscle of pigs and rats. The mechanisms underlying this observation are yet unknown. Based on the previous finding that carnitine increases plasma IGF-1 concentration, we investigated the hypothesis that carnitine down-regulates genes of the UPS by modulation of the of the IGF-1/PI3K/Akt signalling pathway which is an important regulator of UPS activity in muscle. METHODS:Male Sprague-Dawley rats, aged four weeks, were fed either a control diet with a low native carnitine concentration or the same diet supplemented with carnitine (1250 mg/kg diet) for four weeks. Components of the UPS and IGF-1/PI3K/Akt signalling pathway in skeletal muscle were examined. RESULTS:Rats fed the diet supplemented with carnitine had lower mRNA and protein levels of MuRF1, the most important E3 ubiquitin ligase in muscle, decreased concentrations of ubiquitin-protein conjugates in skeletal muscle and higher IGF-1 concentration in plasma than control rats (P < 0.05). Moreover, in skeletal muscle of rats fed the diet supplemented with carnitine there was an activation of the PI3K/Akt signalling pathway, as indicated by increased protein levels of phosphorylated (activated) Akt1 (P < 0.05). CONCLUSION:The present study shows that supplementation of carnitine markedly decreases the expression of MuRF1 and concentrations of ubiquitinated proteins in skeletal muscle of rats, indicating a diminished degradation of myofibrillar proteins by the UPS. The study moreover shows that supplementation of carnitine leads to an activation of the IGF-1/PI3K/Akt signalling pathway which in turn might contribute to the observed down-regulation of MuRF1 and muscle protein ubiquitination. | en |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-100530 | |
dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9022 | |
dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8410 | |
dc.language.iso | de | de_DE |
dc.rights | Namensnennung 3.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | * |
dc.subject | carnitine | en |
dc.subject | rat | en |
dc.subject | muscle | en |
dc.subject | Ubiquitin-proteasome system | en |
dc.subject | IGF-1/PI3K/Akt signalling pathway | en |
dc.subject.ddc | ddc:630 | de_DE |
dc.title | Supplementation of carnitine leads to an activation of the IGF-1/PI3K/Akt signalling pathway and down regulates the E3 ligase MuRF1 in skeletal muscle of rats | en |
dc.type | article | de_DE |
local.affiliation | FB 11 - Medizin | de_DE |
local.opus.fachgebiet | Medizin | de_DE |
local.opus.id | 10053 | |
local.source.freetext | Nutrition & Metabolism 10(1):28 | de_DE |
local.source.uri | https://doi.org/10.1186/1743-7075-10-28 |
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